| http://purl.uniprot.org/citations/3800398 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3800398 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3800398 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/3800398 | http://www.w3.org/2000/01/rdf-schema#comment | "The sesquiterpene cyclase, trichodiene synthetase, has been purified from a supernatant fraction of Fusarium sporotrichioides by hydrophobic interaction, anion exchange, and gel filtration chromatography. Purified enzyme had a specific activity 15-fold higher than that previously reported for preparations of terpene cyclases. Molecular weight determinations by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration chromatography indicated the enzyme to be a dimer with a subunit of Mr 45,000. The requirement of Mg2+ (Km 0.1 mM) for activity could be partially substituted with Mn2+ at a concentration of 0.01 mM, but higher concentrations of Mn2+ were inhibitory. Maximum activity was observed between pH 6.75 and pH 7.75. The Km for farnesyl pyrophosphate was 0.065 microM."xsd:string |
| http://purl.uniprot.org/citations/3800398 | http://purl.org/dc/terms/identifier | "doi:10.1016/0003-9861(86)90386-3"xsd:string |
| http://purl.uniprot.org/citations/3800398 | http://purl.org/dc/terms/identifier | "doi:10.1016/0003-9861(86)90386-3"xsd:string |
| http://purl.uniprot.org/citations/3800398 | http://purl.uniprot.org/core/author | "Hohn T.M."xsd:string |
| http://purl.uniprot.org/citations/3800398 | http://purl.uniprot.org/core/author | "Hohn T.M."xsd:string |
| http://purl.uniprot.org/citations/3800398 | http://purl.uniprot.org/core/author | "Vanmiddlesworth F."xsd:string |
| http://purl.uniprot.org/citations/3800398 | http://purl.uniprot.org/core/author | "Vanmiddlesworth F."xsd:string |
| http://purl.uniprot.org/citations/3800398 | http://purl.uniprot.org/core/date | "1986"xsd:gYear |
| http://purl.uniprot.org/citations/3800398 | http://purl.uniprot.org/core/date | "1986"xsd:gYear |
| http://purl.uniprot.org/citations/3800398 | http://purl.uniprot.org/core/name | "Arch. Biochem. Biophys."xsd:string |
| http://purl.uniprot.org/citations/3800398 | http://purl.uniprot.org/core/name | "Arch. Biochem. Biophys."xsd:string |
| http://purl.uniprot.org/citations/3800398 | http://purl.uniprot.org/core/pages | "756-761"xsd:string |
| http://purl.uniprot.org/citations/3800398 | http://purl.uniprot.org/core/pages | "756-761"xsd:string |
| http://purl.uniprot.org/citations/3800398 | http://purl.uniprot.org/core/title | "Purification and characterization of the sesquiterpene cyclase trichodiene synthetase from Fusarium sporotrichioides."xsd:string |
| http://purl.uniprot.org/citations/3800398 | http://purl.uniprot.org/core/title | "Purification and characterization of the sesquiterpene cyclase trichodiene synthetase from Fusarium sporotrichioides."xsd:string |
| http://purl.uniprot.org/citations/3800398 | http://purl.uniprot.org/core/volume | "251"xsd:string |
| http://purl.uniprot.org/citations/3800398 | http://purl.uniprot.org/core/volume | "251"xsd:string |
| http://purl.uniprot.org/citations/3800398 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3800398 |
| http://purl.uniprot.org/citations/3800398 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3800398 |
| http://purl.uniprot.org/citations/3800398 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3800398 |
| http://purl.uniprot.org/citations/3800398 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/3800398 |
| http://purl.uniprot.org/citations/3800398 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/3800398 |