| http://purl.uniprot.org/citations/38019609 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/38019609 | http://www.w3.org/2000/01/rdf-schema#comment | "Drp1 is a dynamin family GTPase required for mitochondrial and peroxisomal division. Oligomerization increases Drp1 GTPase activity through interactions between neighboring GTPase domains. In cells, Drp1 is regulated by several factors including Drp1 receptors, actin filaments, cardiolipin, and phosphorylation at two sites: S579 and S600. Commonly, phosphorylation of S579 is considered activating, while S600 phosphorylation is considered inhibiting. However, direct effects of phosphorylation on Drp1 GTPase activity have not been investigated in detail. Here, we compare effects of S579 and S600 phosphorylation on purified Drp1, using phosphomimetic mutants and in vitro phosphorylation. Both phosphomimetic mutants are shifted toward smaller oligomers. Both phosphomimetic mutations maintain basal GTPase activity, but eliminate GTPase stimulation by actin and decrease GTPase stimulation by cardiolipin, Mff, and MiD49. Phosphorylation of S579 by Erk2 produces similar effects. When mixed with wildtype Drp1, both S579D and S600D phosphomimetic mutants reduce the actin-stimulated GTPase activity of Drp1-WT. Conversely, a Drp1 mutant (K38A) lacking GTPase activity stimulates Drp1-WT GTPase activity under both basal and actin-stimulated conditions. These results suggest that the effect of S579 phosphorylation is not to activate Drp1 directly. In addition, our results suggest that nearest neighbor interactions within the Drp1 oligomer affect catalytic activity."xsd:string |
| http://purl.uniprot.org/citations/38019609 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e23-11-0427"xsd:string |
| http://purl.uniprot.org/citations/38019609 | http://purl.uniprot.org/core/author | "Liu A."xsd:string |
| http://purl.uniprot.org/citations/38019609 | http://purl.uniprot.org/core/author | "Higgs H.N."xsd:string |
| http://purl.uniprot.org/citations/38019609 | http://purl.uniprot.org/core/author | "Hatch A.L."xsd:string |
| http://purl.uniprot.org/citations/38019609 | http://purl.uniprot.org/core/date | "2024"xsd:gYear |
| http://purl.uniprot.org/citations/38019609 | http://purl.uniprot.org/core/name | "Mol Biol Cell"xsd:string |
| http://purl.uniprot.org/citations/38019609 | http://purl.uniprot.org/core/pages | "ar16"xsd:string |
| http://purl.uniprot.org/citations/38019609 | http://purl.uniprot.org/core/title | "Effects of phosphorylation on Drp1 activation by its receptors, actin, and cardiolipin."xsd:string |
| http://purl.uniprot.org/citations/38019609 | http://purl.uniprot.org/core/volume | "35"xsd:string |
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