| http://purl.uniprot.org/citations/4019411 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/4019411 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/4019411 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/4019411 | http://www.w3.org/2000/01/rdf-schema#comment | "A new enzyme, haloalkane dehalogenase, was isolated from the 1,2-dichloroethane-utilizing bacterium Xanthobacter autotrophicus GJ10. The purified enzyme catalyzed the hydrolytic dehalogenation of n-halogenated C1 to C4 alkanes, including chlorinated, brominated, and iodinated compounds. The highest activity was found with 1,2-dichloroethane, 1,3-dichloropropane, and 1,2-dibromoethane. The enzyme followed Michaelis-Menten kinetics, and the Km for 1,2-dichloroethane was 1.1 mM. Maximum activity was found at pH 8.2 and 37 degrees C. Thiol reagents such as p-chloromercuribenzoate and iodoacetamide rapidly inhibited the enzyme. The protein consists of a single polypeptide chain of a molecular weight of 36,000, and its amino acid composition and N-terminal sequence are given."xsd:string |
| http://purl.uniprot.org/citations/4019411 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.163.2.635-639.1985"xsd:string |
| http://purl.uniprot.org/citations/4019411 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.163.2.635-639.1985"xsd:string |
| http://purl.uniprot.org/citations/4019411 | http://purl.uniprot.org/core/author | "Janssen D.B."xsd:string |
| http://purl.uniprot.org/citations/4019411 | http://purl.uniprot.org/core/author | "Janssen D.B."xsd:string |
| http://purl.uniprot.org/citations/4019411 | http://purl.uniprot.org/core/author | "Witholt B."xsd:string |
| http://purl.uniprot.org/citations/4019411 | http://purl.uniprot.org/core/author | "Witholt B."xsd:string |
| http://purl.uniprot.org/citations/4019411 | http://purl.uniprot.org/core/author | "Keuning S."xsd:string |
| http://purl.uniprot.org/citations/4019411 | http://purl.uniprot.org/core/author | "Keuning S."xsd:string |
| http://purl.uniprot.org/citations/4019411 | http://purl.uniprot.org/core/date | "1985"xsd:gYear |
| http://purl.uniprot.org/citations/4019411 | http://purl.uniprot.org/core/date | "1985"xsd:gYear |
| http://purl.uniprot.org/citations/4019411 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/4019411 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/4019411 | http://purl.uniprot.org/core/pages | "635-639"xsd:string |
| http://purl.uniprot.org/citations/4019411 | http://purl.uniprot.org/core/pages | "635-639"xsd:string |
| http://purl.uniprot.org/citations/4019411 | http://purl.uniprot.org/core/title | "Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10."xsd:string |
| http://purl.uniprot.org/citations/4019411 | http://purl.uniprot.org/core/title | "Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10."xsd:string |
| http://purl.uniprot.org/citations/4019411 | http://purl.uniprot.org/core/volume | "163"xsd:string |
| http://purl.uniprot.org/citations/4019411 | http://purl.uniprot.org/core/volume | "163"xsd:string |
| http://purl.uniprot.org/citations/4019411 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/4019411 |
| http://purl.uniprot.org/citations/4019411 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/4019411 |
| http://purl.uniprot.org/citations/4019411 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/4019411 |