| http://purl.uniprot.org/citations/4044584 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/4044584 | http://www.w3.org/2000/01/rdf-schema#comment | "Platelets have previously been shown to contain a membrane skeleton that is composed of actin filaments, actin-binding protein, and three membrane glycoproteins (GP), GP Ib, GP Ia, and a minor glycoprotein of Mr = 250,000. The present study was designed to determine how the membrane glycoproteins were linked to actin filaments. Unstimulated platelets were lysed with Triton X-100, and the membrane skeleton was isolated on sucrose density gradients or by high-speed centrifugation. The association of the membrane glycoproteins with the actin filaments was disrupted when actin-binding protein was hydrolyzed by activity of the Ca2+-dependent protease, which was active in platelet lysates upon addition of Ca2+ in the absence of leupeptin. Similarly, activation of the Ca2+-dependent protease in intact platelets by the addition of a platelet agonist also caused the membrane glycoproteins to dissociate from the membrane skeleton. Affinity-purified actin-binding protein antibodies immunoprecipitated the membrane glycoproteins from platelet lysates in which actin filaments had been removed by DNase I-induced depolymerization and high-speed centrifugation. These results demonstrate that actin-binding protein links actin filaments of the platelet membrane skeleton to three plasma membrane glycoproteins and that filaments are released from their attachment site when actin-binding protein is hydrolyzed by the Ca2+-dependent protease within intact platelets during platelet activation."xsd:string |
| http://purl.uniprot.org/citations/4044584 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(17)38972-x"xsd:string |
| http://purl.uniprot.org/citations/4044584 | http://purl.uniprot.org/core/author | "Fox J.E."xsd:string |
| http://purl.uniprot.org/citations/4044584 | http://purl.uniprot.org/core/date | "1985"xsd:gYear |
| http://purl.uniprot.org/citations/4044584 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/4044584 | http://purl.uniprot.org/core/pages | "11970-11977"xsd:string |
| http://purl.uniprot.org/citations/4044584 | http://purl.uniprot.org/core/title | "Identification of actin-binding protein as the protein linking the membrane skeleton to glycoproteins on platelet plasma membranes."xsd:string |
| http://purl.uniprot.org/citations/4044584 | http://purl.uniprot.org/core/volume | "260"xsd:string |
| http://purl.uniprot.org/citations/4044584 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/4044584 |
| http://purl.uniprot.org/citations/4044584 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/4044584 |
| http://purl.uniprot.org/uniprot/P21333#attribution-0172D38D87FDFD930CB67E7895D31125 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/4044584 |