http://purl.uniprot.org/citations/6090391 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/6090391 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/6090391 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
http://purl.uniprot.org/citations/6090391 | http://www.w3.org/2000/01/rdf-schema#comment | "The genes for alkaline protease (apr[BamP]) and neutral protease (npr[BamP]) from Bacillus amyloliquefaciens have been isolated and expressed in Bacillus subtilis. The DNA sequences of apr[BamP] and npr[BamP] revealed, in each case, the presence of a large open reading frame. The inferred amino acid sequence of either gene contained a signal sequence and an additional polypeptide sequence ('pro' sequence) preceding the mature protein. Based on DNA sequence, the start point of translation has been identified as amino acid residue - 107 for apr[BamP] and -221 for npr[BamP]. To demonstrate that the start point of translation of apr[BamP] in vivo is probably at codon -107, codon -103 (AAA) was changed to an ochre (TAA) by site-directed mutagenesis. Alkaline protease was produced from this ochre mutant derivative of apr[BamP] only when the host strain was Su+. The presence of a pro sequence may be common to all of the secreted proteases from bacilli."xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.159.3.811-819.1984"xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.159.3.811-819.1984"xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/author | "Filpula D."xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/author | "Filpula D."xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/author | "Thompson L.D."xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/author | "Thompson L.D."xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/author | "Rhodes C."xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/author | "Rhodes C."xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/author | "Nagle J."xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/author | "Nagle J."xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/author | "Vasantha N."xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/author | "Vasantha N."xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/author | "Banner C."xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/author | "Banner C."xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/date | "1984"xsd:gYear |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/date | "1984"xsd:gYear |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/pages | "811-819"xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/pages | "811-819"xsd:string |
http://purl.uniprot.org/citations/6090391 | http://purl.uniprot.org/core/title | "Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein."xsd:string |