http://purl.uniprot.org/citations/6130093 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/6130093 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/6130093 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
http://purl.uniprot.org/citations/6130093 | http://www.w3.org/2000/01/rdf-schema#comment | "The regulatory nucleotide guanosine 5'-diphosphate, 3'-diphosphate (ppGpp) and its precursor guanosine 5'-triphosphate, 3'-diphosphate (pppGpp) are accumulated during stringent response in bacterial cells. The enzyme pppGpp-5'-phosphohydrolase, which catalyzes the conversion of pppGpp to ppGpp, was partially purified from Escherichia coli. It has Mr = 140,000 and an apparent Km of 0.11 mM for pppGpp. It requires Mg2+ and a monovalent cation. NH4+ is preferred over K+, while Na+ is inactive. The enzyme does not hydrolyze GTP, ATP, pppApp, or ppGpp. It is also not effectively inhibited by these nucleotides. pppGpp-5'-phosphohydrolase hydrolyzes the 3'-monophosphate analog pppGp equally well (apparent Km of 0.13 mM), yielding the recently identified MS III nucleotide (ppGp). pppGpp-5'-phosphohydrolase does not have RNA 5'-terminal gamma-phosphatase activity; however, 5'-terminal phosphates are released by pppGpp-5'-phosphohydrolase when the GTP-terminated RNA chains are first converted into oligonucleotides by RNase A treatment. pppGpp-5'-phosphohydrolase was found to actively hydrolyze the dinucleotide fragment pppGpNp but exhibited very low activity toward longer chain fragments. The 3'-unphosphorylated dinucleotide pppGpN was, however, not hydrolyzed. The ability of pppGpp-5'-phosphohydrolase to hydrolyze pppGpp, pppGp, and pppGpNp, but not pppG and pppGpN, indicates that pppGpp-5'-phosphohydrolase is rather nonspecific toward the 3'-OH substitutions of the substrates although a free, unsubstituted phosphate group at the 3'-OH position is essential."xsd:string |
http://purl.uniprot.org/citations/6130093 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)33039-4"xsd:string |
http://purl.uniprot.org/citations/6130093 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)33039-4"xsd:string |
http://purl.uniprot.org/citations/6130093 | http://purl.uniprot.org/core/author | "Hara A."xsd:string |
http://purl.uniprot.org/citations/6130093 | http://purl.uniprot.org/core/author | "Hara A."xsd:string |
http://purl.uniprot.org/citations/6130093 | http://purl.uniprot.org/core/author | "Sy J."xsd:string |
http://purl.uniprot.org/citations/6130093 | http://purl.uniprot.org/core/author | "Sy J."xsd:string |
http://purl.uniprot.org/citations/6130093 | http://purl.uniprot.org/core/date | "1983"xsd:gYear |
http://purl.uniprot.org/citations/6130093 | http://purl.uniprot.org/core/date | "1983"xsd:gYear |
http://purl.uniprot.org/citations/6130093 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
http://purl.uniprot.org/citations/6130093 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
http://purl.uniprot.org/citations/6130093 | http://purl.uniprot.org/core/pages | "1678-1683"xsd:string |
http://purl.uniprot.org/citations/6130093 | http://purl.uniprot.org/core/pages | "1678-1683"xsd:string |
http://purl.uniprot.org/citations/6130093 | http://purl.uniprot.org/core/title | "Guanosine 5'-triphosphate, 3'-diphosphate 5'-phosphohydrolase. Purification and substrate specificity."xsd:string |
http://purl.uniprot.org/citations/6130093 | http://purl.uniprot.org/core/title | "Guanosine 5'-triphosphate, 3'-diphosphate 5'-phosphohydrolase. Purification and substrate specificity."xsd:string |
http://purl.uniprot.org/citations/6130093 | http://purl.uniprot.org/core/volume | "258"xsd:string |
http://purl.uniprot.org/citations/6130093 | http://purl.uniprot.org/core/volume | "258"xsd:string |
http://purl.uniprot.org/citations/6130093 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/6130093 |
http://purl.uniprot.org/citations/6130093 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/6130093 |
http://purl.uniprot.org/citations/6130093 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/6130093 |
http://purl.uniprot.org/citations/6130093 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/6130093 |
http://purl.uniprot.org/citations/6130093 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/6130093 |