| http://purl.uniprot.org/citations/6334084 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/6334084 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/6334084 | http://www.w3.org/2000/01/rdf-schema#comment | "Human renal dipeptidase has been concentrated from kidneys by homogenization, 1-butanol solubilization, and (NH4)2SO4 fractionation. Final purification was achieved by high-pressure liquid chromatography followed by affinity chromatography. The enzyme appeared to be homogeneous by polyacrylamide gel electrophoresis, and its molecular weight was estimated to be 220,000 by analytical high-pressure liquid chromatography. The molecular weight of human urinary dipeptidase was estimated by agarose gel filtration to be 218,000. Dissociation of human renal dipeptidase in sodium dodecyl sulfate-polyacrylamide gel electrophoresis produced a single polypeptide (Mr 59,000). These results suggest that the native enzyme contains four subunits of Mr 59,000. Analysis of the peptidase for zinc content gave 3.9 g atoms of zinc/mol of enzyme which supports the suggestion of a 4-subunit structure. Carbohydrate analyses of the purified human dipeptidase demonstrated that it was not a glycoprotein, a characteristic that distinguishes it from porcine and rat renal dipeptidase. beta-Lactamase activity of the purified human enzyme was demonstrated by measuring its activity against the two beta-lactam antibiotics, imipenem and SCH 29482. Kinetic analyses indicated that both antibiotics undergo enzyme-catalyzed hydrolysis at rates which could produce inactivation of the antibiotics within the human kidney. The beta-lactamase inhibitor, cilastatin, demonstrated reversible competitive inhibition of the peptidase-catalyzed hydrolysis of both antibiotics with the same Ki of 0.7 microM."xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(17)42642-1"xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(17)42642-1"xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/author | "Campbell B.J."xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/author | "Campbell B.J."xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/author | "Burks M."xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/author | "Burks M."xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/author | "Forrester L.J."xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/author | "Forrester L.J."xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/author | "Zahler W.L."xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/author | "Zahler W.L."xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/date | "1984"xsd:gYear |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/date | "1984"xsd:gYear |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/pages | "14586-14590"xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/pages | "14586-14590"xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/title | "Beta-lactamase activity of purified and partially characterized human renal dipeptidase."xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/title | "Beta-lactamase activity of purified and partially characterized human renal dipeptidase."xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/volume | "259"xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://purl.uniprot.org/core/volume | "259"xsd:string |
| http://purl.uniprot.org/citations/6334084 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/6334084 |
| http://purl.uniprot.org/citations/6334084 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/6334084 |