| http://purl.uniprot.org/citations/6350298 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/6350298 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/6350298 | http://www.w3.org/2000/01/rdf-schema#comment | "Dihydrofolate reductase specified by plasmid R483 from a trimethoprim-resistant strain of Escherichia coli has been purified 2,000-fold to homogeneity using dye-ligand chromatography, gel filtration, and polyacrylamide gel electrophoresis. The protein migrated as a single band on nondenaturing polyacrylamide gel electrophoresis and had a specific activity of 250 mumol/mg min(-1). The molecular weight was estimated to be 32,000 by gel filtration and 39,000 by Ferguson analysis of polyacrylamide gel electrophoresis. When subjected to electrophoresis in the presence of sodium dodecyl sulfate, the protein migrated as a single 19,000-molecular weight species, a fact that suggests that the native enzyme is a dimer of similar or identical subunits. Antibody specific for R483-encoded dihydrofolate reductase did not cross-react with dihydrofolate reductase encoded by plasmid R67, T4 phage, E. coli RT500, or mouse L1210 leukemia cells. The amino acid sequence of the first 34 NH2-terminal residues suggests that the R483 plasmid dihydrofolate reductase is more closely related to the chromosomal dihydrofolate reductase than is the enzyme coded by plasmid R67."xsd:string |
| http://purl.uniprot.org/citations/6350298 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(17)44369-9"xsd:string |
| http://purl.uniprot.org/citations/6350298 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(17)44369-9"xsd:string |
| http://purl.uniprot.org/citations/6350298 | http://purl.uniprot.org/core/author | "Stone D."xsd:string |
| http://purl.uniprot.org/citations/6350298 | http://purl.uniprot.org/core/author | "Stone D."xsd:string |
| http://purl.uniprot.org/citations/6350298 | http://purl.uniprot.org/core/author | "Novak P."xsd:string |
| http://purl.uniprot.org/citations/6350298 | http://purl.uniprot.org/core/author | "Novak P."xsd:string |
| http://purl.uniprot.org/citations/6350298 | http://purl.uniprot.org/core/author | "Burchall J.J."xsd:string |
| http://purl.uniprot.org/citations/6350298 | http://purl.uniprot.org/core/author | "Burchall J.J."xsd:string |
| http://purl.uniprot.org/citations/6350298 | http://purl.uniprot.org/core/date | "1983"xsd:gYear |
| http://purl.uniprot.org/citations/6350298 | http://purl.uniprot.org/core/date | "1983"xsd:gYear |
| http://purl.uniprot.org/citations/6350298 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/6350298 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/6350298 | http://purl.uniprot.org/core/pages | "10956-10959"xsd:string |
| http://purl.uniprot.org/citations/6350298 | http://purl.uniprot.org/core/pages | "10956-10959"xsd:string |
| http://purl.uniprot.org/citations/6350298 | http://purl.uniprot.org/core/title | "R plasmid dihydrofolate reductase with a dimeric subunit structure."xsd:string |
| http://purl.uniprot.org/citations/6350298 | http://purl.uniprot.org/core/title | "R plasmid dihydrofolate reductase with a dimeric subunit structure."xsd:string |
| http://purl.uniprot.org/citations/6350298 | http://purl.uniprot.org/core/volume | "258"xsd:string |
| http://purl.uniprot.org/citations/6350298 | http://purl.uniprot.org/core/volume | "258"xsd:string |
| http://purl.uniprot.org/citations/6350298 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/6350298 |
| http://purl.uniprot.org/citations/6350298 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/6350298 |
| http://purl.uniprot.org/citations/6350298 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/6350298 |
| http://purl.uniprot.org/citations/6350298 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/6350298 |