| http://purl.uniprot.org/citations/6359161 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/6359161 | http://www.w3.org/2000/01/rdf-schema#comment | "The peptide mating pheromone alpha-factor and the hydrolytic enzyme invertase (beta-D-fructofuranoside fructohydrolase, EC 3.2.1.26) are processed from larger precursor proteins during their secretion from yeast cells (Saccharomyces cerevisiae). An in-frame fusion of the structural genes for these two proteins was constructed by connecting the 5'-flanking region and prepro-leader portion of the coding sequence of the alpha-factor gene (MF alpha 1) to a large fragment of the invertase gene (SUC2) lacking its 5'-flanking region and the coding information for the first four amino acids of its signal sequence. Sites that have been implicated in normal proteolytic processing of the alpha-factor precursor have been retained in this construction. The chimeric gene directs synthesis of a high level of active invertase that is secreted efficiently into the periplasmic space, permitting cell growth on sucrose-containing media. This extracellular invertase appears to contain no prepro-alpha-factor sequences. The initial intracellular product is, however, a hybrid protein that can be detected either by treatment of the cells with the drug tunicamycin or by blockage of secretion in a temperature-conditional secretion-defective mutant (sec18). Therefore, prior to its efficient proteolytic removal, the alpha-factor portion of the hybrid protein apparently provides the necessary information for efficient export of the substantially larger protein invertase. Similar to MF alpha 1, the MF alpha 1-SUC2 fusion is expressed in alpha haploids at levels 65-75 times higher than in a haploids or in a/alpha diploids; also, high-level expression is eliminated in mat alpha 1 mutants but not in mat alpha 2 mutants. Unlike expression of SUC2, expression of the fusion is not affected by glucose concentration. Hence, the 5'-flanking region present in the fusion (about 950 base pairs) is sufficient to confer alpha cell-specific expression to the hybrid gene."xsd:string |
| http://purl.uniprot.org/citations/6359161 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.80.23.7080"xsd:string |
| http://purl.uniprot.org/citations/6359161 | http://purl.uniprot.org/core/author | "Emr S.D."xsd:string |
| http://purl.uniprot.org/citations/6359161 | http://purl.uniprot.org/core/author | "Schekman R."xsd:string |
| http://purl.uniprot.org/citations/6359161 | http://purl.uniprot.org/core/author | "Thorner J."xsd:string |
| http://purl.uniprot.org/citations/6359161 | http://purl.uniprot.org/core/author | "Flessel M.C."xsd:string |
| http://purl.uniprot.org/citations/6359161 | http://purl.uniprot.org/core/date | "1983"xsd:gYear |
| http://purl.uniprot.org/citations/6359161 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/6359161 | http://purl.uniprot.org/core/pages | "7080-7084"xsd:string |
| http://purl.uniprot.org/citations/6359161 | http://purl.uniprot.org/core/title | "An MF alpha 1-SUC2 (alpha-factor-invertase) gene fusion for study of protein localization and gene expression in yeast."xsd:string |
| http://purl.uniprot.org/citations/6359161 | http://purl.uniprot.org/core/volume | "80"xsd:string |
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