| http://purl.uniprot.org/citations/6403940 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/6403940 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/6403940 | http://www.w3.org/2000/01/rdf-schema#comment | "We have synthesized 175-nucleotide-long probes for the DNA of human histocompatibility antigens HLA-DR alpha and beta by extending on poly(A)+ mRNA from B-cell lines with short synthetic deoxyribonucleotide primers complementary to the predicted nucleotide sequence of the NH2 terminus of both polypeptides. The synthesis of the probe for the alpha-chain DNA was a two-step process starting with 11-mers which were extended by dideoxynucleotide chain termination experiments to a 20-mer of predicted sequence. The synthesized 20-mer was then used to generate a 175-nucleotide cDNA probe which was shown to encode the appropriate amino acids for the alpha chain and was used to select a human genomic DNA clone containing the coding sequences for HLA-DR alpha. For the beta polypeptide an 18-mer homologous to the NH2-terminal sequence of a cDNA clone from another B-cell line was used to extend on poly(A)+ mRNA isolated from a B-cell line. Preliminary sequence analysis of a 175-base-long extension product indicates a match of the cDNA sequence to the published sequence of a clone for HLA-DR beta. Information from these extension experiments helps to establish the sensitivity and specificity of the primer extension method."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.80.6.1531"xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.80.6.1531"xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Lemaux P.G."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Lemaux P.G."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Weissman S.M."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Weissman S.M."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Peterlin B.M."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Peterlin B.M."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Cohen S.N."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Cohen S.N."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Sood A.K."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Sood A.K."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Das H.K."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Das H.K."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Biro P.A."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Biro P.A."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "von Gabain A."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "von Gabain A."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Erlich H.A."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Erlich H.A."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Lawrance S.K."xsd:string |
| http://purl.uniprot.org/citations/6403940 | http://purl.uniprot.org/core/author | "Lawrance S.K."xsd:string |