http://purl.uniprot.org/citations/6406228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/6406228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/6406228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
http://purl.uniprot.org/citations/6406228 | http://www.w3.org/2000/01/rdf-schema#comment | "2-Aminoethylphosphonate aminotransferase has been purified to homogeneity with a yield of 15% from cell extracts of Pseudomonas aeruginosa. The molecular weight of the enzyme was estimated by gel filtration to be 65000 +/-2000. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis yielded a molecular weight of 16500 +/-1000, suggesting a tetrameric model for this protein. The absorption spectrum exhibits maxima at 280 nm, 335 nm and 415 nm which are characteristic of a pyridoxal-phosphate-dependent enzyme: 4 mol of pyridoxal 5'-phosphate/mol of enzyme have been found. This aminotransferase catalyzes the transfer of the amino group of 2-aminoethylphosphonate (ciliatine) to pyruvate to give 2-phosphonoacetaldehyde and alanine. A pH optimum between 8.5-9 and an activity increasing from 30 degrees C to 50 degrees C have been observed. The reaction follows Michaelis-Menten kinetics with Km values of 3.85 mM and 3.5 mM for ciliatine and pyruvate respectively. This enzyme shows a very high specificity since ciliatine and pyruvate are the only amino donor and acceptor respectively. Methyl, ethyl and propylphosphonic acids are better competitors towards ciliatine than their alpha-amino derivatives. 3-Aminopropylphosphonate, the higher homologue of ciliatine, is recognized neither as a substrate nor as an inhibitor. The enzyme activity is significantly affected by carbonyl reagents and by HgCl2. Transamination of 2-aminoethylphosphonate is the first step of a double-step pathway which leads to the cleavage of its C-P bond."xsd:string |
http://purl.uniprot.org/citations/6406228 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1983.tb07436.x"xsd:string |
http://purl.uniprot.org/citations/6406228 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1983.tb07436.x"xsd:string |
http://purl.uniprot.org/citations/6406228 | http://purl.uniprot.org/core/author | "Cassaigne A."xsd:string |
http://purl.uniprot.org/citations/6406228 | http://purl.uniprot.org/core/author | "Cassaigne A."xsd:string |
http://purl.uniprot.org/citations/6406228 | http://purl.uniprot.org/core/author | "Dumora C."xsd:string |
http://purl.uniprot.org/citations/6406228 | http://purl.uniprot.org/core/author | "Dumora C."xsd:string |
http://purl.uniprot.org/citations/6406228 | http://purl.uniprot.org/core/author | "Lacoste A.-M."xsd:string |
http://purl.uniprot.org/citations/6406228 | http://purl.uniprot.org/core/author | "Lacoste A.-M."xsd:string |
http://purl.uniprot.org/citations/6406228 | http://purl.uniprot.org/core/date | "1983"xsd:gYear |
http://purl.uniprot.org/citations/6406228 | http://purl.uniprot.org/core/date | "1983"xsd:gYear |
http://purl.uniprot.org/citations/6406228 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
http://purl.uniprot.org/citations/6406228 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
http://purl.uniprot.org/citations/6406228 | http://purl.uniprot.org/core/pages | "119-125"xsd:string |
http://purl.uniprot.org/citations/6406228 | http://purl.uniprot.org/core/pages | "119-125"xsd:string |
http://purl.uniprot.org/citations/6406228 | http://purl.uniprot.org/core/title | "Purification and properties of 2-aminoethylphosphonate:pyruvate aminotransferase from Pseudomonas aeruginosa."xsd:string |
http://purl.uniprot.org/citations/6406228 | http://purl.uniprot.org/core/title | "Purification and properties of 2-aminoethylphosphonate:pyruvate aminotransferase from Pseudomonas aeruginosa."xsd:string |
http://purl.uniprot.org/citations/6406228 | http://purl.uniprot.org/core/volume | "133"xsd:string |
http://purl.uniprot.org/citations/6406228 | http://purl.uniprot.org/core/volume | "133"xsd:string |
http://purl.uniprot.org/citations/6406228 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/6406228 |
http://purl.uniprot.org/citations/6406228 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/6406228 |
http://purl.uniprot.org/citations/6406228 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/6406228 |