| http://purl.uniprot.org/citations/6416294 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/6416294 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/6416294 | http://www.w3.org/2000/01/rdf-schema#comment | "An improved procedure for the isolation of the C1 inhibitor (C1-INH) component of human complement is reported. Following preliminary steps to remove plasminogen, fibrinogen, and aggregated material, three conventional chromatographic steps are used to isolate C1-INH in high (70%) overall yield. An extinction coefficient (E 1%, 1 cm 280nm) of 3.60 has been determined. The isolated protein exhibits a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with a mobility corresponding to an apparent molecular weight (Mr) of 105 000. After removal of carbohydrate, the protein shows an increased mobility, corresponding to an apparent Mr of 78 000. A total carbohydrate content of 33% has been calculated, and from this and the size of the deglycosylated polypeptide, a true molecular weight of 116 000 was estimated. Further analysis of the carbohydrate has indicated a galactose:mannose ratio of 2:1 and approximately equimolar amounts of N-acetylglucosamine and N-acetylgalactosamine. This composition is unusual for a plasma protein and suggests that much of the carbohydrate is contained in linkages other than the typical N-glycosidic structures. Values found for the amino acid composition are compared with those reported previously. The amino-terminal sequence (40 residues) of C1-INH is also reported. Asparagine lies at the amino terminus. Neither high-performance liquid chromatography of the released phenylthiohydantoin derivative nor back-hydrolysis of the thiazolinone permitted identification of the residue contained at position 3. The sequence around this position is compatible, however, with an N-glycosidic linkage to residue 3.(ABSTRACT TRUNCATED AT 250 WORDS)"xsd:string |
| http://purl.uniprot.org/citations/6416294 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00290a019"xsd:string |
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| http://purl.uniprot.org/citations/6416294 | http://purl.uniprot.org/core/author | "Harrison R.A."xsd:string |
| http://purl.uniprot.org/citations/6416294 | http://purl.uniprot.org/core/author | "Harrison R.A."xsd:string |
| http://purl.uniprot.org/citations/6416294 | http://purl.uniprot.org/core/date | "1983"xsd:gYear |
| http://purl.uniprot.org/citations/6416294 | http://purl.uniprot.org/core/date | "1983"xsd:gYear |
| http://purl.uniprot.org/citations/6416294 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/6416294 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/6416294 | http://purl.uniprot.org/core/pages | "5001-5007"xsd:string |
| http://purl.uniprot.org/citations/6416294 | http://purl.uniprot.org/core/pages | "5001-5007"xsd:string |
| http://purl.uniprot.org/citations/6416294 | http://purl.uniprot.org/core/title | "Human C1 inhibitor: improved isolation and preliminary structural characterization."xsd:string |
| http://purl.uniprot.org/citations/6416294 | http://purl.uniprot.org/core/title | "Human C1 inhibitor: improved isolation and preliminary structural characterization."xsd:string |
| http://purl.uniprot.org/citations/6416294 | http://purl.uniprot.org/core/volume | "22"xsd:string |
| http://purl.uniprot.org/citations/6416294 | http://purl.uniprot.org/core/volume | "22"xsd:string |
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| http://purl.uniprot.org/citations/6416294 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/6416294 |
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| http://purl.uniprot.org/citations/6416294 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/6416294 |
| http://purl.uniprot.org/uniprot/P05155 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/6416294 |
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| http://purl.uniprot.org/uniprot/#_E9KL26-mappedCitation-6416294 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/6416294 |