| http://purl.uniprot.org/citations/6546753 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/6546753 | http://www.w3.org/2000/01/rdf-schema#comment | "Polysomes containing cytosolic malic enzyme mRNA and malic enzyme nascent chains were complexed with specific antibodies and purified by chromatography on protein A-Sepharose. When poly(A+) mRNA derived from the immunoselected polysomes was translated in vitro, full length malic enzyme (subunit Mr = 58,000) accounted for a significant fraction (approximately 20%) of the polypeptides synthesized. Double-stranded cDNA, synthesized using partially purified malic enzyme mRNA as a template, was inserted into pBR 322 and cloned. Twenty-five candidate malic enzyme cDNA clones were identified by differential hybridization. Four clones were studied further and each of these was shown to have malic enzyme cDNA sequences by hybrid-selected translation and specific immunoprecipitation. Plasmid pME1, which contains a 1400-base pair insert, hybridized to two mouse liver malic enzyme mRNAs with lengths of 2300 and 3500 bases. Similar analyses were performed on liver mRNAs isolated from MOD-1 mutant mice which lack cytosolic malic enzyme activity. These Northern blots disclosed a pair of aberrantly large malic enzyme mRNAs with lengths of 2800 and 4000 bases. Furthermore, anti-malic enzyme antibodies exclusively precipitated a polypeptide translation product with a Mr of 77,000 when MOD-1 mRNA was used to direct in vitro protein synthesis. Thus, it is possible that MOD-1 malic enzyme mRNA contains an additional polypeptide coding sequence. The translation of such a sequence might disrupt enzyme function and/or markedly decrease enzyme stability. The malic enzyme cDNA probe was also employed to demonstrate that the induction of malic enzyme in the livers of previously starved mice that were fed a high carbohydrate, fat-free diet was controlled pretranslationally by a parallel modulation of the malic enzyme mRNA concentration."xsd:string |
| http://purl.uniprot.org/citations/6546753 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(17)43697-0"xsd:string |
| http://purl.uniprot.org/citations/6546753 | http://purl.uniprot.org/core/author | "Rubin C.S."xsd:string |
| http://purl.uniprot.org/citations/6546753 | http://purl.uniprot.org/core/author | "Sul H.S."xsd:string |
| http://purl.uniprot.org/citations/6546753 | http://purl.uniprot.org/core/author | "Brown M.L."xsd:string |
| http://purl.uniprot.org/citations/6546753 | http://purl.uniprot.org/core/author | "Wise L.S."xsd:string |
| http://purl.uniprot.org/citations/6546753 | http://purl.uniprot.org/core/date | "1984"xsd:gYear |
| http://purl.uniprot.org/citations/6546753 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/6546753 | http://purl.uniprot.org/core/pages | "555-559"xsd:string |
| http://purl.uniprot.org/citations/6546753 | http://purl.uniprot.org/core/title | "Cloning of cDNA sequences for murine malic enzyme and the identification of aberrantly large malic enzyme mRNA in MOD-1 null mice."xsd:string |
| http://purl.uniprot.org/citations/6546753 | http://purl.uniprot.org/core/volume | "259"xsd:string |
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