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http://purl.uniprot.org/citations/6548461http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/6548461http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/6548461http://www.w3.org/2000/01/rdf-schema#comment"Polysome immuno-adsorption, with immunoglobulin G directed against two 3-methylcholanthrene-induced mouse liver cytochrome P-450 proteins, was used to enrich mRNA from 3-methylcholanthrene-treated C57BL/6N mouse liver. cDNA transcribed from the P-450-enriched mRNA was then cloned into the Okayama-Berg vector. Two cDNA classes were detected upon differential screening of the clone bank with [32P]cDNA derived from 3-methylcholanthrene-induced immuno-enriched versus control mRNA. Several representatives of these two classes were judged to be near full length by comparison with their corresponding mRNA mobilities on denaturing agarose gels. A continuous reading-frame near the 5' end of one cDNA class (P1-450) corresponds to a protein having 15 of 17 residues the same as the published N-terminal sequence of rat P-450c. A continuous reading frame near the 5' end of the other class (P3-450) corresponds exactly to the first 25 amino acids of the published N-terminal sequence of rat P-450d. The P1-450 cDNA is at least 700 bp longer than the P3-450 cDNA. Heteroduplex analysis and Southern blot hybridization demonstrate that these mRNAs share approx. 1100 bp of sequence homology. Genomic P1-450 and P3-450 clones were isolated from a gene library constructed from C57BL/6N mouse liver DNA. By heteroduplex analysis with the corresponding cDNA, the P1-450 gene spans about 6 kb and the P3-450 gene about 7 kb. The intron-exon patterns are very similar, with the second and seventh exons being much larger than the other five. The 3' terminal exon of P1-450 is about 500 bp longer than that of P3-450. These data suggest that both P1-450 and P3-450 have diverged from a common ancestral gene."xsd:string
http://purl.uniprot.org/citations/6548461http://purl.org/dc/terms/identifier"doi:10.1016/0378-1119(84)90057-x"xsd:string
http://purl.uniprot.org/citations/6548461http://purl.org/dc/terms/identifier"doi:10.1016/0378-1119(84)90057-x"xsd:string
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/author"Kimura S."xsd:string
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/author"Kimura S."xsd:string
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/author"Gonzalez F.J."xsd:string
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/author"Gonzalez F.J."xsd:string
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/author"Nebert D.W."xsd:string
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/author"Nebert D.W."xsd:string
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/author"McKenzie P.I."xsd:string
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/author"McKenzie P.I."xsd:string
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/date"1984"xsd:gYear
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/date"1984"xsd:gYear
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/name"Gene"xsd:string
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/name"Gene"xsd:string
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/pages"281-292"xsd:string
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/pages"281-292"xsd:string
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/title"Isolation and characterization of full-length mouse cDNA and genomic clones of 3-methylcholanthrene-inducible cytochrome P1-450 and P3-450."xsd:string
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/title"Isolation and characterization of full-length mouse cDNA and genomic clones of 3-methylcholanthrene-inducible cytochrome P1-450 and P3-450."xsd:string
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/volume"29"xsd:string
http://purl.uniprot.org/citations/6548461http://purl.uniprot.org/core/volume"29"xsd:string
http://purl.uniprot.org/citations/6548461http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/6548461
http://purl.uniprot.org/citations/6548461http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/6548461