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http://purl.uniprot.org/citations/6548747http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/6548747http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/6548747http://www.w3.org/2000/01/rdf-schema#comment"We have constructed a cDNA-expression library of approximately 100,000 members from embryonic chicken smooth-muscle mRNA using the plasmid-expression vectors pUC8 and pUC9. Using an immunological screening procedure and 32P-labeled cDNA probes, we have identified and isolated clones encoding smooth-muscle tropomyosin. Plasmid pSMT-10 (approximately 1100 base pairs) was found to hybrid-select mRNA for smooth-muscle alpha-tropomyosin. DNA-sequence analysis revealed that pSMT-10 contained the entire coding region for alpha-tropomyosin and portions of the 5'- and 3'-untranslated regions. Comparison of the derived amino acid sequence of smooth-muscle alpha-tropomyosin with known skeletal-muscle (rabbit and chicken) and platelet (equine) sequences revealed extensive homology between the various proteins. The smooth-muscle tropomyosin shows the greatest sequence divergence from the skeletal-muscle tropomyosins at the COOH-terminal region. In contrast, the smooth-muscle tropomyosin is most homologous to the platelet tropomyosin at the COOH-terminal end. The relationship of the various tropomyosin sequences to function (e.g. interactions with troponin) are considered."xsd:string
http://purl.uniprot.org/citations/6548747http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(18)89867-2"xsd:string
http://purl.uniprot.org/citations/6548747http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(18)89867-2"xsd:string
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/author"Helfman D.M."xsd:string
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/author"Helfman D.M."xsd:string
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/author"Hughes S.H."xsd:string
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/author"Hughes S.H."xsd:string
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/author"Feramisco J.R."xsd:string
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/author"Feramisco J.R."xsd:string
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/author"Ricci W.M."xsd:string
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/author"Ricci W.M."xsd:string
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/date"1984"xsd:gYear
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/date"1984"xsd:gYear
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/pages"14136-14143"xsd:string
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/pages"14136-14143"xsd:string
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/title"Isolation and sequence of a cDNA clone that contains the entire coding region for chicken smooth-muscle alpha-tropomyosin."xsd:string
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/title"Isolation and sequence of a cDNA clone that contains the entire coding region for chicken smooth-muscle alpha-tropomyosin."xsd:string
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/volume"259"xsd:string
http://purl.uniprot.org/citations/6548747http://purl.uniprot.org/core/volume"259"xsd:string
http://purl.uniprot.org/citations/6548747http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/6548747
http://purl.uniprot.org/citations/6548747http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/6548747