| http://purl.uniprot.org/citations/6586719 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/6586719 | http://www.w3.org/2000/01/rdf-schema#comment | "Dependence on the salt concentration of the activity of microsome-bound arylsulfatase C [EC 3.1.6.1] from rat liver was examined. The activity increased with increasing salt concentration in the reaction medium in the whole pH range tested. This effect can be explained by the dependence of the reaction rate on the surface pH and the surface concentration of the ionic substrate. The dependence on salt concentration of the activity of the microsome-bound arylsulfatase C and the pH-dependences of Vmax and Km of the enzyme were used for the estimation of pH at the microsomal surface. The two values of the surface pH (surface potential) and the salt concentration were applied to the Gouy-Chapman equation. The value of -0.39 +/-0.08 X 10(-3) elementary charge/A2 was obtained as the surface charge density in the vicinity of the microsome-bound arylsulfatase C. This was smaller than the over-all value for microsomes (-1.08 +/-0.04 X 10(-3) elementary charge/A2; Masamoto, K. (1982) J. Biochem. 92, 365-371). This suggests that the anion concentration in the vicinity of the enzyme on microsomes is lower than that in the bulk aqueous phase and is higher than the average value at the microsomal surface when the salt concentration is low."xsd:string |
| http://purl.uniprot.org/citations/6586719 | http://purl.org/dc/terms/identifier | "doi:10.1093/oxfordjournals.jbchem.a134661"xsd:string |
| http://purl.uniprot.org/citations/6586719 | http://purl.uniprot.org/core/author | "Masamoto K."xsd:string |
| http://purl.uniprot.org/citations/6586719 | http://purl.uniprot.org/core/date | "1984"xsd:gYear |
| http://purl.uniprot.org/citations/6586719 | http://purl.uniprot.org/core/name | "J Biochem"xsd:string |
| http://purl.uniprot.org/citations/6586719 | http://purl.uniprot.org/core/pages | "715-719"xsd:string |
| http://purl.uniprot.org/citations/6586719 | http://purl.uniprot.org/core/title | "Dependence on surface pH and surface substrate concentration of activity of microsome-bound arylsulfatase C and the surface charge density in the vicinity of the enzyme."xsd:string |
| http://purl.uniprot.org/citations/6586719 | http://purl.uniprot.org/core/volume | "95"xsd:string |
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| http://purl.uniprot.org/uniprot/P15589#attribution-29C9EF285582E085CB8C822B70894CA5 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/6586719 |
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| http://purl.uniprot.org/uniprot/P15589 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/6586719 |
| http://purl.uniprot.org/uniprot/G3V9E5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/6586719 |