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http://purl.uniprot.org/citations/6803844http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/6803844http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/6803844http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Citation
http://purl.uniprot.org/citations/6803844http://www.w3.org/2000/01/rdf-schema#comment"Alanine-glyoxylate aminotransferase and 2-aminobutyrate aminotransferase were co-purified from rat kidney to a single protein (about 500-fold purified from the homogenate). The activity ratios of alanine-glyoxylate aminotransferase to 2-aminobutyrate aminotransferase were constant during co-purification steps suggesting the 2-aminobutyrate aminotransferase activity was catalysed by only alanine-glyoxylate aminotransferase. The molecular weight of the enzyme was estimated to be approx. 213 000, 220 000 and 236 000 by analytical ultracentrifugation, Sephadex G-150 gel filtration and sucrose density gradient centrifugation, respectively. From the polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate, the enzyme consisted of four apparently similar subunits having a molecular weight of approx. 56 000. The enzyme was almost specific to L-alanine and L-2-aminobutyrate as amino donor and to glyoxylate, pyruvate and 2-oxobutyrate as amino acceptor. The enzyme was identified with rat liver alanine-glyoxylate aminotransferase isoenzyme 2 but not with rat liver alanine-glyoxylate aminotransferase isoenzyme 1 from Ouchterlony double diffusion analysis. Absorption spectra and some kinetic properties of the enzyme were clarified."xsd:string
http://purl.uniprot.org/citations/6803844http://purl.org/dc/terms/identifier"doi:10.1016/0304-4165(82)90054-x"xsd:string
http://purl.uniprot.org/citations/6803844http://purl.org/dc/terms/identifier"doi:10.1016/0304-4165(82)90054-x"xsd:string
http://purl.uniprot.org/citations/6803844http://purl.org/dc/terms/identifier"doi:10.1016/0304-4165(82)90054-x"xsd:string
http://purl.uniprot.org/citations/6803844http://purl.uniprot.org/core/author"Okuno E."xsd:string
http://purl.uniprot.org/citations/6803844http://purl.uniprot.org/core/author"Okuno E."xsd:string
http://purl.uniprot.org/citations/6803844http://purl.uniprot.org/core/author"Kido R."xsd:string
http://purl.uniprot.org/citations/6803844http://purl.uniprot.org/core/author"Kido R."xsd:string
http://purl.uniprot.org/citations/6803844http://purl.uniprot.org/core/author"Minatogawa Y."xsd:string
http://purl.uniprot.org/citations/6803844http://purl.uniprot.org/core/author"Minatogawa Y."xsd:string
http://purl.uniprot.org/citations/6803844http://purl.uniprot.org/core/date"1982"xsd:gYear
http://purl.uniprot.org/citations/6803844http://purl.uniprot.org/core/date"1982"xsd:gYear
http://purl.uniprot.org/citations/6803844http://purl.uniprot.org/core/name"Biochim. Biophys. Acta"xsd:string
http://purl.uniprot.org/citations/6803844http://purl.uniprot.org/core/name"Biochim. Biophys. Acta"xsd:string
http://purl.uniprot.org/citations/6803844http://purl.uniprot.org/core/pages"97-104"xsd:string
http://purl.uniprot.org/citations/6803844http://purl.uniprot.org/core/pages"97-104"xsd:string
http://purl.uniprot.org/citations/6803844http://purl.uniprot.org/core/title"Co-purification of alanine-glyoxylate aminotransferase with 2-aminobutyrate aminotransferase in rat kidney."xsd:string
http://purl.uniprot.org/citations/6803844http://purl.uniprot.org/core/title"Co-purification of alanine-glyoxylate aminotransferase with 2-aminobutyrate aminotransferase in rat kidney."xsd:string
http://purl.uniprot.org/citations/6803844http://purl.uniprot.org/core/volume"715"xsd:string
http://purl.uniprot.org/citations/6803844http://purl.uniprot.org/core/volume"715"xsd:string
http://purl.uniprot.org/citations/6803844http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/6803844
http://purl.uniprot.org/citations/6803844http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/6803844