http://purl.uniprot.org/citations/6945134 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/6945134 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/6945134 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
http://purl.uniprot.org/citations/6945134 | http://www.w3.org/2000/01/rdf-schema#comment | "Eight strains of Clostridium absonum grown in the presence of 4 . 10(-4) M deoxycholate contained both NADP-dependent 7 alpha- and 7 beta-hydroxysteroid dehydrogenase activities. In one strain studied in detail, significant amounts of NADP-dependent 7 alpha- and 7 beta-hydroxysteroid dehydrogenase and NAD-dependent 7 alpha-hydroxysteroid dehydrogenase activities were demonstrated only when cells were grown in the presence of deoxycholate or chenodeoxycholate, both optimal at 4 . 10(-4) M. When the bile salt was deleted from the medium, only a trace of 7 alpha-hydroxysteroid dehydrogenase was present and 7 beta-hydroxysteroid dehydrogenase was absent. Other bile salts including cholate, ursodeoxycholate and keto bile salts were less effective as inducers. Addition of cholate to medium already containing deoxycholate at a suboptimal concentration enhanced the induction, while addition of ursodeoxycholate suppressed the induction. Further enhancement of 7 alpha- and 7 beta-hydroxysteroid dehydrogenase could be obtained by additions of deoxycholate (up to a total of 6 . 10(-4) M) during the growth of the organisms (in log phase). As enzyme enhancement is blocked by addition of rifampicin to the medium, the authors conclude that the enzymes are bile salt-inducible. Growth curve studies revealed an optimal enzyme yield at a harvest time of approx. 6-9 h. We have preliminarily characterized several inducible enzyme components: an NADP-dependent 7 beta-hydroxysteroid dehydrogenase as well as both NAD- and NADP-dependent 7 alpha-hydroxysteroid dehydrogenases."xsd:string |
http://purl.uniprot.org/citations/6945134 | http://purl.org/dc/terms/identifier | "doi:10.1016/0005-2760(81)90011-4"xsd:string |
http://purl.uniprot.org/citations/6945134 | http://purl.org/dc/terms/identifier | "doi:10.1016/0005-2760(81)90011-4"xsd:string |
http://purl.uniprot.org/citations/6945134 | http://purl.uniprot.org/core/author | "MacDonald I.A."xsd:string |
http://purl.uniprot.org/citations/6945134 | http://purl.uniprot.org/core/author | "MacDonald I.A."xsd:string |
http://purl.uniprot.org/citations/6945134 | http://purl.uniprot.org/core/author | "Roach P.D."xsd:string |
http://purl.uniprot.org/citations/6945134 | http://purl.uniprot.org/core/author | "Roach P.D."xsd:string |
http://purl.uniprot.org/citations/6945134 | http://purl.uniprot.org/core/date | "1981"xsd:gYear |
http://purl.uniprot.org/citations/6945134 | http://purl.uniprot.org/core/date | "1981"xsd:gYear |
http://purl.uniprot.org/citations/6945134 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
http://purl.uniprot.org/citations/6945134 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
http://purl.uniprot.org/citations/6945134 | http://purl.uniprot.org/core/pages | "262-269"xsd:string |
http://purl.uniprot.org/citations/6945134 | http://purl.uniprot.org/core/pages | "262-269"xsd:string |
http://purl.uniprot.org/citations/6945134 | http://purl.uniprot.org/core/title | "Bile induction of 7 alpha- and 7 beta-hydroxysteroid dehydrogenases in Clostridium absonum."xsd:string |
http://purl.uniprot.org/citations/6945134 | http://purl.uniprot.org/core/title | "Bile induction of 7 alpha- and 7 beta-hydroxysteroid dehydrogenases in Clostridium absonum."xsd:string |
http://purl.uniprot.org/citations/6945134 | http://purl.uniprot.org/core/volume | "665"xsd:string |
http://purl.uniprot.org/citations/6945134 | http://purl.uniprot.org/core/volume | "665"xsd:string |
http://purl.uniprot.org/citations/6945134 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/6945134 |
http://purl.uniprot.org/citations/6945134 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/6945134 |
http://purl.uniprot.org/citations/6945134 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/6945134 |
http://purl.uniprot.org/citations/6945134 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/6945134 |
http://purl.uniprot.org/citations/6945134 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/6945134 |