| http://purl.uniprot.org/citations/6997293 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/6997293 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/6997293 | http://www.w3.org/2000/01/rdf-schema#comment | "The tRNA-modifying enzyme, S-adenosylmethionine: tRNA (uridine-5)-methyltransferase, has been purified essentially to homogeneity from an Escherichia coli strain containing an elevated level of this enzyme. A rapid, efficient method has been developed for the purification, consisting of polyethyleneimine precipitation to remove nucleic acids, followed by phosphocellulose and Blue Sepharose affinity chromatography. The enzyme is a single polypeptide chain of molecular weight 42,000. It has a pH optimum of 8.4, a Km of 12.5 microM for S-adenyosyl-L-methionine, and a Km of 1.1 microM for wheat germ tRNAGly1. The ability of the enzyme to methylate a variety of tRNA substrates including prokaryotic, eukaryotic, mitochondrial, and chloroplastic tRNAs has been characterized."xsd:string |
| http://purl.uniprot.org/citations/6997293 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(19)70646-2"xsd:string |
| http://purl.uniprot.org/citations/6997293 | http://purl.uniprot.org/core/author | "Greenberg R."xsd:string |
| http://purl.uniprot.org/citations/6997293 | http://purl.uniprot.org/core/author | "Dudock B."xsd:string |
| http://purl.uniprot.org/citations/6997293 | http://purl.uniprot.org/core/date | "1980"xsd:gYear |
| http://purl.uniprot.org/citations/6997293 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/6997293 | http://purl.uniprot.org/core/pages | "8296-8302"xsd:string |
| http://purl.uniprot.org/citations/6997293 | http://purl.uniprot.org/core/title | "Isolation and chracterization of m5U-methyltransferase from Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/6997293 | http://purl.uniprot.org/core/volume | "255"xsd:string |
| http://purl.uniprot.org/citations/6997293 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/6997293 |
| http://purl.uniprot.org/citations/6997293 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/6997293 |
| http://purl.uniprot.org/citations/6997293 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/6997293 |
| http://purl.uniprot.org/citations/6997293 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/6997293 |
| http://purl.uniprot.org/enzyme/2.1.1.35 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/6997293 |
| http://purl.uniprot.org/uniprot/P23003#attribution-E854227EFD7F5A3C4211EFE23A3038FA | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/6997293 |