| http://purl.uniprot.org/citations/7038398 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7038398 | http://www.w3.org/2000/01/rdf-schema#comment | "In the preceding paper of this series (Dujardin et al. 1980 a) we described general methods of selecting and genetically characterizing suppressor mutations that restore the respiratory capacity of mit-mitochondrial mutations. Two dominant nuclear (NAM1-1 and NAM2-1) and one mitochondrial (mim2-1) suppressors are more extensively studied in this paper. We have analysed the action spectrum of these suppressors on 433 mit-mutations located in various mitochondrial genes and found that they preferentially alleviate the effects of mutations located within intron open reading frames of the cob-box gene. We conclude that these suppressors permit the maturation of cytochrome b mRNA by restoring the synthesis of intron encoded protein(s) catalytically involved in splicing i.e. mRNA-maturase(s) (cf. Lazowska et al. 1980). NAM1-1 is allele specific and gene non-specific; it suppresses mutations located within different introns. NAM2-1 and mim2-1 are intron-specific: they suppress mutations all located in the same (box7) intron of the cob-box gene. Analyses of cytochrome absorption spectra and mitochondrial translation products of cells in which the suppressors are associated with various other mit-mutations show that the suppressors restore cytochrome b and/or cytochrome oxidase (cox I) synthesis, as expected from their growth phenotype. This suppression is, however, only partial: some new polypeptides characteristic of the mit-mutations can be still detected in the presence of suppressor. Interestingly enough when box7 specific suppressors NAM2-1 and mim2-1 are associated with a complete cob-box deletion (leading to a total deficiency of cytochrome b and oxidase) partial restoration of cox I synthesis is observed while cytochrome b is still totally absent. These results show that in strains carrying NAM2-1 or mim2-1 the presence of cytochrome b gene is no longer required for the expression of the oxi3 gene pointing out to the possibility of a mutational switch-on of silent genes, whether mitochondrial, mim2-1, or nuclear, NAM2-1. This switch-on would permit the synthesis of an active maturase acting as a substitute for the box7 maturase in order to splice the cytochrome b and oxidase mRNAs."xsd:string |
| http://purl.uniprot.org/citations/7038398 | http://purl.org/dc/terms/identifier | "doi:10.1007/bf00352529"xsd:string |
| http://purl.uniprot.org/citations/7038398 | http://purl.uniprot.org/core/author | "Dujardin G."xsd:string |
| http://purl.uniprot.org/citations/7038398 | http://purl.uniprot.org/core/author | "Slonimski P.P."xsd:string |
| http://purl.uniprot.org/citations/7038398 | http://purl.uniprot.org/core/author | "Groudinsky O."xsd:string |
| http://purl.uniprot.org/citations/7038398 | http://purl.uniprot.org/core/date | "1981"xsd:gYear |
| http://purl.uniprot.org/citations/7038398 | http://purl.uniprot.org/core/name | "Mol Gen Genet"xsd:string |
| http://purl.uniprot.org/citations/7038398 | http://purl.uniprot.org/core/pages | "493-503"xsd:string |
| http://purl.uniprot.org/citations/7038398 | http://purl.uniprot.org/core/title | "Long range control circuits within mitochondria and between nucleus and mitochondria. II. Genetic and biochemical analyses of suppressors which selectively alleviate the mitochondrial intron mutations."xsd:string |
| http://purl.uniprot.org/citations/7038398 | http://purl.uniprot.org/core/volume | "184"xsd:string |
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| http://purl.uniprot.org/uniprot/P11325 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/7038398 |