| http://purl.uniprot.org/citations/7504525 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7504525 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7504525 | http://www.w3.org/2000/01/rdf-schema#comment | "We have previously identified hsp56, a protein component of steroid receptor complexes, as an FK506 binding protein [Yem et al. (1992) J. Biol. Chem. 267, 2868-2871]. We now report that hsp56 is also found to be a major immunophilin in chicken thymus, by virtue of binding to FK506-Affi-Gel-10 as well as positive cross-reactivity with a polyclonal antiserum directed against human hsp56. Limited digests of purified chicken hsp56 with endoproteinase Lys C result in the production of a unique polypeptide having a mass of about 17 kDa (p17), as judged by Western blotting. Peptide mapping provided additional proof that p17 is a fragment which comprises the entire FK506 binding domain I of chicken hsp56, terminating with an Arg-Lys which might represent a processing site. Binding of radiolabeled dihydro FK506 to p17 is saturable with a calculated KD of 42 nM. Since size exclusion chromatography of drug-p17 complexes indicates that the active species is a homodimer with a mass of 30-40 kDa, the stoichiometry calculated for the drug-protein complex is approximately 1:1. Furthermore, unlike FKBP-12, chicken p17 bound to FK506 does not bind to calcineurin-calmodulin complexes. This work demonstrates the excision of a domain from an hsp56 protein that is active in binding FK506 and functionally distinct from FKBP-12, a protein of similar size and structure."xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00210a004"xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00210a004"xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/author | "Heinrikson R.L."xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/author | "Heinrikson R.L."xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/author | "Reardon I.M."xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/author | "Reardon I.M."xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/author | "Deibel M.R. Jr."xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/author | "Leone J.W."xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/author | "Leone J.W."xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/author | "Yem A.W."xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/author | "Yem A.W."xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/author | "Deibel M.R. Jr"xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/pages | "12571-12576"xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/pages | "12571-12576"xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/title | "An active FK506-binding domain of 17,000 daltons is isolated following limited proteolysis of chicken thymus hsp56."xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/title | "An active FK506-binding domain of 17,000 daltons is isolated following limited proteolysis of chicken thymus hsp56."xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/volume | "32"xsd:string |
| http://purl.uniprot.org/citations/7504525 | http://purl.uniprot.org/core/volume | "32"xsd:string |