| http://purl.uniprot.org/citations/7531687 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7531687 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7531687 | http://www.w3.org/2000/01/rdf-schema#comment | "Nitric oxide (NO) is a messenger molecule with diverse functions throughout the body. The inducible type of nitric oxide synthase (NOS) is considered to be a key molecule in the immune responses to bacteria, parasites, and tumors, and its gene expression is regulated by cytokines. We isolated 3 overlapping partial inducible NOS cDNA clones from a human glioblastoma cell line A-172 induced by IL-1, TNF-alpha, and IFN-gamma. The 3,963-bp human glioblastoma inducible NOS cDNA contained the longest open reading frame of 3,459 bp, which encoded a polypeptide of 1,153 amino acids with a calculated molecular mass of 131 kDa. This human inducible NOS possessed consensus recognition sites for the cofactors FMN, FAD, and NADPH and calmodulin recognition sites, and displayed 48.1% sequence identity with the endothelial type, 43.1% with the neuronal type, and 99.3% with the inducible type from hepatocytes, and 99.9% with the inducible type from chondrocytes and adenocarcinoma. An expression plasmid consisting of pSG5 expression vector and cDNA containing the entire putative coding sequence was constructed and transfected into COS-1 cells. COS-1 cells showed nitric oxide synthase activity together with a 130 kDa immunoreactive band on Western blot analysis."xsd:string |
| http://purl.uniprot.org/citations/7531687 | http://purl.org/dc/terms/identifier | "doi:10.1093/oxfordjournals.jbchem.a124563"xsd:string |
| http://purl.uniprot.org/citations/7531687 | http://purl.org/dc/terms/identifier | "doi:10.1093/oxfordjournals.jbchem.a124563"xsd:string |
| http://purl.uniprot.org/citations/7531687 | http://purl.uniprot.org/core/author | "Esumi H."xsd:string |
| http://purl.uniprot.org/citations/7531687 | http://purl.uniprot.org/core/author | "Esumi H."xsd:string |
| http://purl.uniprot.org/citations/7531687 | http://purl.uniprot.org/core/author | "Zeniya M."xsd:string |
| http://purl.uniprot.org/citations/7531687 | http://purl.uniprot.org/core/author | "Zeniya M."xsd:string |
| http://purl.uniprot.org/citations/7531687 | http://purl.uniprot.org/core/author | "Hokari A."xsd:string |
| http://purl.uniprot.org/citations/7531687 | http://purl.uniprot.org/core/author | "Hokari A."xsd:string |
| http://purl.uniprot.org/citations/7531687 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/7531687 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/7531687 | http://purl.uniprot.org/core/name | "J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/7531687 | http://purl.uniprot.org/core/name | "J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/7531687 | http://purl.uniprot.org/core/pages | "575-581"xsd:string |
| http://purl.uniprot.org/citations/7531687 | http://purl.uniprot.org/core/pages | "575-581"xsd:string |
| http://purl.uniprot.org/citations/7531687 | http://purl.uniprot.org/core/title | "Cloning and functional expression of human inducible nitric oxide synthase (NOS) cDNA from a glioblastoma cell line A-172."xsd:string |
| http://purl.uniprot.org/citations/7531687 | http://purl.uniprot.org/core/title | "Cloning and functional expression of human inducible nitric oxide synthase (NOS) cDNA from a glioblastoma cell line A-172."xsd:string |
| http://purl.uniprot.org/citations/7531687 | http://purl.uniprot.org/core/volume | "116"xsd:string |
| http://purl.uniprot.org/citations/7531687 | http://purl.uniprot.org/core/volume | "116"xsd:string |
| http://purl.uniprot.org/citations/7531687 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7531687 |
| http://purl.uniprot.org/citations/7531687 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7531687 |
| http://purl.uniprot.org/citations/7531687 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7531687 |
| http://purl.uniprot.org/citations/7531687 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7531687 |