http://purl.uniprot.org/citations/7537849 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/7537849 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/7537849 | http://www.w3.org/2000/01/rdf-schema#comment | "The SHC proteins have been implicated in insulin receptor (IR) signaling. In this study, we used the sensitive two-hybrid assay of protein-protein interaction to demonstrate that SHC interacts directly with the IR. The interaction is mediated by SHC amino acids 1 to 238 and is therefore independent of the Src homology 2 domain. The interaction is dependent upon IR autophosphorylation, since the interaction is eliminated by mutation of the IR ATP-binding site. In addition, mutational analysis of the Asn-Pro-Glu-Tyr (NPEY) motif within the juxtamembrane domain of the IR showed the importance of the Asn, Pro, and Tyr residues to both SHC and IR substrate 1 (IRS-1) binding. We conclude that SHC interacts directly with the IR and that phosphorylation of Tyr-960 within the IR juxtamembrane domain is necessary for efficient interaction. This interaction is highly reminiscent of that of IRS-1 with the IR, and we show that the SHC IR-binding domain can substitute for that of IRS-1 in yeast and COS cells. We identify a homologous region within the IR-binding domains of SHC and IRS-1, which we term the SAIN (SHC and IRS-1 NPXY-binding) domain, which may explain the basis of these interactions. The SAIN domain appears to represent a novel motif which is able to interact with autophosphorylated receptors such as the IR."xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.15.5.2500"xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.15.5.2500"xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/author | "He W."xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/author | "He W."xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/author | "Gustafson T.A."xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/author | "Gustafson T.A."xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/author | "O'Neill T.J."xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/author | "O'Neill T.J."xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/author | "Craparo A."xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/author | "Craparo A."xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/author | "Schaub C.D."xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/author | "Schaub C.D."xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/pages | "2500-2508"xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/pages | "2500-2508"xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/title | "Phosphotyrosine-dependent interaction of SHC and insulin receptor substrate 1 with the NPEY motif of the insulin receptor via a novel non-SH2 domain."xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/title | "Phosphotyrosine-dependent interaction of SHC and insulin receptor substrate 1 with the NPEY motif of the insulin receptor via a novel non-SH2 domain."xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/volume | "15"xsd:string |
http://purl.uniprot.org/citations/7537849 | http://purl.uniprot.org/core/volume | "15"xsd:string |