| http://purl.uniprot.org/citations/7539802 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7539802 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7539802 | http://www.w3.org/2000/01/rdf-schema#comment | "The c-kit-encoded tyrosine kinase receptor for stem cell factor (Kit/SCFR) is crucial for the development of hematopoietic cells, melanoblasts, and germ cells. Ligand stimulation of Kit/SCFR leads to receptor dimerization and autophosphorylation on tyrosine residues. We recently showed, that protein kinase C (PKC) acts in an SCF-stimulated negative feedback loop, which controls Kit/SCFR tyrosine kinase activity and modulates the cellular responses to SCF (Blume-Jensen, P., Siegbahn, A., Stabel, S., Heldin, C.-H., and Rönnstrand, L. (1993) EMBO J. 12, 4199-4209). We present here the identification of the major phosphorylation sites for PKC in Kit/SCFR. Two serine residues in the kinase insert, Ser-741 and Ser-746, are PKC-dependent phosphorylation sites in vivo and account for all phosphorylation by PKC in vitro. Together they comprise more than 60% of the total SCF-stimulated receptor phosphorylation in living cells and 85-90% of its phosphorylation in resting cells. Two additional serine residues, Ser-821 close to the major tyrosine autophosphorylation site in the kinase domain and Ser-959 in the carboxyl terminus are SCF-stimulated PKC-dependent phosphorylation sites. However, they are not phosphorylated directly by PKC-alpha in vitro. Both specific receptor tyrosine autophosphorylation and specific receptor-associated phosphatidylinositide 3'-kinase activity was increased approximately 2-fold in response to SCF in PAE cells stably expressing Kit/SCFR(S741A/S746A). Furthermore, the kinase activity of Kit/SCFR(S741A/S746A) toward an exogenous substrate was increased, which was reflected as a decreased Km and an increased Vmax, in accordance with the negative regulatory role of PKC on Kit/SCFR signaling."xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.270.23.14192"xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.270.23.14192"xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/author | "Wernstedt C."xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/author | "Wernstedt C."xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/author | "Heldin C.H."xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/author | "Heldin C.H."xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/author | "Ronnstrand L."xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/author | "Ronnstrand L."xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/author | "Blume-Jensen P."xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/author | "Blume-Jensen P."xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/pages | "14192-14200"xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/pages | "14192-14200"xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/title | "Identification of the major phosphorylation sites for protein kinase C in kit/stem cell factor receptor in vitro and in intact cells."xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/title | "Identification of the major phosphorylation sites for protein kinase C in kit/stem cell factor receptor in vitro and in intact cells."xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/volume | "270"xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://purl.uniprot.org/core/volume | "270"xsd:string |
| http://purl.uniprot.org/citations/7539802 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7539802 |
| http://purl.uniprot.org/citations/7539802 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7539802 |