| http://purl.uniprot.org/citations/7541240 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7541240 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7541240 | http://www.w3.org/2000/01/rdf-schema#comment | "A novel selective calcium channel antagonist peptide, SNX-325, has been isolated from the venom of the spider Segestria florentina. The peptide was isolated using as bioassays the displacement of radioiodinated omega-conopeptide SNX-230 (MVIIC) from rat brain synaptosomal membranes, as well as the inhibition of the barium current through cloned expressed calcium channels in oocytes. The primary sequence of SNX-325 is GSCIESGKSCTHSRSMKNGLCCPKSRCNCRQIQHRHDYLGKRKYSCRCS, which is a novel amino acid sequence. Solid-phase synthesis resulted in a peptide that is chromatographically identical with the native peptide and which has the same configuration of cysteine residues as the spider venom peptide omega-Aga-IVa [Mintz, I. M., et al., (1992) Nature 355, 827-829]. At micromolar concentrations, SNX-325 is an inhibitor of most calcium, but not sodium or potassium, currents. At nanomolar concentrations, SNX-325 is a selective blocker of the cloned expressed class B (N-type), but not class C (cardiac L), A, or E, calcium channels. SNX-325 is approximately equipotent with the N-channel selective omega-conopeptides (GVIA and MVIIA as well as closely related synthetic derivatives) in blocking the potassium induced release of tritiated norepinephrine from hippocampal slices (IC50s, 0.1-0.5 nM) and in blocking the barium current through cloned expressed N-channels in oocytes (IC50s 3-30 nM). By contrast, SNX-325 is 4-5 orders of magnitude less potent than is SNX-111 (synthetic MVIIA) at displacing radioiodinated SNX-111 from rat brain synaptosomal membranes. SNX-325 will be a useful comparative tool in further defining the function and pharmacology of the N- and possibly other types of high-voltage activated calcium channels."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00026a015"xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00026a015"xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Newcomb R."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Newcomb R."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Lau K."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Lau K."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Fox J."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Fox J."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Bell J.R."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Bell J.R."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Chung D."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Chung D."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Palma A."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Palma A."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Nadasdi L."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Nadasdi L."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Cong R."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Cong R."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Gaur S."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Gaur S."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Horne B."xsd:string |
| http://purl.uniprot.org/citations/7541240 | http://purl.uniprot.org/core/author | "Horne B."xsd:string |