| http://purl.uniprot.org/citations/7543777 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7543777 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7543777 | http://www.w3.org/2000/01/rdf-schema#comment | "We have generated DNA encoding the mature form of ovine interleukin 2 (IL-2) by polymerase chain reaction (PCR) using primers complementary to sequences at the 5' and 3' ends of human, murine and bovine IL-2 cDNA. The predicted PCR product of 400 bp was ligated into the yeast Ty-P1 galactose-inducible expression vector pOGS40 which was used to transform yeast spheroplasts. The fusion protein, with a Factor Xa proteolytic cleavage site between ovine IL-2 and the P1 fusion partner, was expressed from galactose-induced transformed yeast. P1:IL-2 fusion protein, which self-assembles into virus-like particles (VLPs) due to the interaction of the P1 protein, was purified from lysates of mechanically disrupted yeast by centrifugation on a discontinuous sucrose gradient. Fusion protein was detected in Western blot analysis with polyclonal antisera raised to recombinant bovine IL-2. Soluble recombinant ovine IL-2 was released from the P1 fusion protein by cleavage with Factor Xa enzyme. After purification recombinant ovine IL-2 was functionally active as shown by its ability to support the proliferation of Con A-activated T cells and was capable of generating maedi visna virus-specific cytotoxic T cells from primed precursor cells. The availability of recombinant ovine IL-2 will greatly help the analysis of the specificity of pathogen-specific cells in the sheep."xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.org/dc/terms/identifier | "doi:10.1006/cyto.1995.0025"xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.org/dc/terms/identifier | "doi:10.1006/cyto.1995.0025"xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/author | "Roy D."xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/author | "Roy D."xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/author | "Hunt P."xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/author | "Hunt P."xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/author | "Bujdoso R."xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/author | "Bujdoso R."xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/author | "Sargan D."xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/author | "Sargan D."xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/author | "Blacklaws B."xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/author | "Blacklaws B."xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/author | "McConnell I."xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/author | "McConnell I."xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/author | "Williamson M.L."xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/author | "Williamson M.L."xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/name | "Cytokine"xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/name | "Cytokine"xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/pages | "223-231"xsd:string |
| http://purl.uniprot.org/citations/7543777 | http://purl.uniprot.org/core/pages | "223-231"xsd:string |