| http://purl.uniprot.org/citations/7568118 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7568118 | http://www.w3.org/2000/01/rdf-schema#comment | "The mechanism of mitogen-activated protein (MAP) kinase activation by pertussis toxin-sensitive Gi-coupled receptors is known to involve the beta gamma subunits of heterotrimeric G proteins (G beta gamma), p21ras activation, and an as-yet-unidentified tyrosine kinase. To investigate the mechanism of G beta gamma-stimulated p21ras activation, G beta gamma-mediated tyrosine phosphorylation was examined by overexpressing G beta gamma or alpha 2-C10 adrenergic receptors (ARs) that couple to Gi in COS-7 cells. Immunoprecipitation of phosphotyrosine-containing proteins revealed a 2-to 3-fold increase in the phosphorylation of two proteins of approximately 50 kDa (designated as p52) in G beta gamma-transfected cells or in alpha 2-C10 AR-transfected cells stimulated with the agonist UK-14304. The latter response was pertussis toxin sensitive. These proteins (p52) were also specifically immunoprecipitated with anti-Shc antibodies and comigrated with two Shc proteins, 46 and 52 kDa. The G beta gamma- or alpha 2-C10 AR-stimulated p52 (Shc) phosphorylation was inhibited by coexpression of the carboxyl terminus of beta-adrenergic receptor kinase (a G beta gamma-binding pleckstrin homology domain peptide) or by the tyrosine kinase inhibitors genistein and herbimycin A, but not by a dominant negative mutant of p21ras. Worthmannin, a specific inhibitor of phosphatidylinositol 3-kinase (PI3K) inhibited phosphorylation of p52 (Shc), implying involvement of PI3K. These results suggest that G beta gamma-stimulated Shc phosphorylation represents an early step in the pathway leading to p21ras activation, similar to the mechanism utilized by growth factor tyrosine kinase receptors."xsd:string |
| http://purl.uniprot.org/citations/7568118 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.92.20.9284"xsd:string |
| http://purl.uniprot.org/citations/7568118 | http://purl.uniprot.org/core/author | "Lefkowitz R.J."xsd:string |
| http://purl.uniprot.org/citations/7568118 | http://purl.uniprot.org/core/author | "Touhara K."xsd:string |
| http://purl.uniprot.org/citations/7568118 | http://purl.uniprot.org/core/author | "van Biesen T."xsd:string |
| http://purl.uniprot.org/citations/7568118 | http://purl.uniprot.org/core/author | "Hawes B.E."xsd:string |
| http://purl.uniprot.org/citations/7568118 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7568118 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/7568118 | http://purl.uniprot.org/core/pages | "9284-9287"xsd:string |
| http://purl.uniprot.org/citations/7568118 | http://purl.uniprot.org/core/title | "G protein beta gamma subunits stimulate phosphorylation of Shc adapter protein."xsd:string |
| http://purl.uniprot.org/citations/7568118 | http://purl.uniprot.org/core/volume | "92"xsd:string |
| http://purl.uniprot.org/citations/7568118 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7568118 |
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