| http://purl.uniprot.org/citations/7592594 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7592594 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7592594 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/7592594 | http://www.w3.org/2000/01/rdf-schema#comment | "Deoxyhypusine synthase catalyzes the formation of deoxyhypusine residue on the eIF-5A precursor using spermidine as the substrate. We have purified deoxyhypusine synthase from Neurospora crassa to apparent homogeneity (Tao, Y., and Chen, K. Y. (1995) J. Biol. Chem. 270, 383-386). We have now cloned and characterized the deoxyhypusine synthase cDNA using a reverse genetic approach. Conceptual translation of the nucleotide sequence of the cloned 1258-base pair cDNA revealed an open reading frame containing 353 amino acids with a predicted M(r) of 38,985. The deoxyhypusine synthase cDNA was subcloned into the expression vector pQE60 to produce a 40,000-dalton recombinant protein on SDS-PAGE which exhibited deoxyhypusine synthase activity. A GenBank search showed that the Neurospora deoxyhypusine synthase cDNA possessed significant sequence homology to a previously uncharacterized yeast sequence (accession number U00061 (1994)). The yeast sequence encodes a protein of 387 amino acids that shows 69% of total amino acid identity and 80% of total amino acid similarity to the Neurospora enzyme. Sequence alignment and hydropathy analysis suggest that the yeast sequence represents deoxyhypusine synthase."xsd:string |
| http://purl.uniprot.org/citations/7592594 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.270.41.23984"xsd:string |
| http://purl.uniprot.org/citations/7592594 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.270.41.23984"xsd:string |
| http://purl.uniprot.org/citations/7592594 | http://purl.uniprot.org/core/author | "Tao Y."xsd:string |
| http://purl.uniprot.org/citations/7592594 | http://purl.uniprot.org/core/author | "Tao Y."xsd:string |
| http://purl.uniprot.org/citations/7592594 | http://purl.uniprot.org/core/author | "Chen K.Y."xsd:string |
| http://purl.uniprot.org/citations/7592594 | http://purl.uniprot.org/core/author | "Chen K.Y."xsd:string |
| http://purl.uniprot.org/citations/7592594 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7592594 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7592594 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7592594 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7592594 | http://purl.uniprot.org/core/pages | "23984-23987"xsd:string |
| http://purl.uniprot.org/citations/7592594 | http://purl.uniprot.org/core/pages | "23984-23987"xsd:string |
| http://purl.uniprot.org/citations/7592594 | http://purl.uniprot.org/core/title | "Molecular cloning and functional expression of Neurospora deoxyhypusine synthase cDNA and identification of yeast deoxyhypusine synthase cDNA."xsd:string |
| http://purl.uniprot.org/citations/7592594 | http://purl.uniprot.org/core/title | "Molecular cloning and functional expression of Neurospora deoxyhypusine synthase cDNA and identification of yeast deoxyhypusine synthase cDNA."xsd:string |
| http://purl.uniprot.org/citations/7592594 | http://purl.uniprot.org/core/volume | "270"xsd:string |
| http://purl.uniprot.org/citations/7592594 | http://purl.uniprot.org/core/volume | "270"xsd:string |
| http://purl.uniprot.org/citations/7592594 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7592594 |
| http://purl.uniprot.org/citations/7592594 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7592594 |
| http://purl.uniprot.org/citations/7592594 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7592594 |
| http://purl.uniprot.org/citations/7592594 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7592594 |
| http://purl.uniprot.org/citations/7592594 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7592594 |