| http://purl.uniprot.org/citations/7592904 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7592904 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7592904 | http://www.w3.org/2000/01/rdf-schema#comment | "Initial rolling of circulating neutrophils on a blood vessel wall prior to adhesion and transmigration to damaged tissue is dependent upon P-selectin expressed on endothelial cells and its specific neutrophil receptor, the P-selectin glycoprotein ligand-1 (PSGL-1). Pretreatment of neutrophils, HL60 cells, or a recombinant fucosylated soluble form of PSGL-1 (sPSGL-1.T7) with the cobra venom metalloproteinase, mocarhagin, completely abolished binding to purified P-selectin in a time-dependent and EDTA- and diisopropyl fluorophosphate-inhibitable manner consistent with mocarhagin selectively cleaving PSGL-1. A polyclonal antibody against the N-terminal peptide Gln-1-Glu-15 of mature PSGL-1 immunoprecipitated sPSGL-1.T7 but not sPSGL-1.T7 treated with mocarhagin, indicating that the mocarhagin cleavage site was near the N terminus. A single mocarhagin cleavage site between Tyr-10 and Asp-11 of mature PSGL-1 was determined by N-terminal sequencing of mocarhagin fragments of sPSGL-1.T7 and is within a highly negatively charged amino acid sequence 1-QATEYEYLDY decreases DFLPETEPPE, containing three tyrosine residues that are consensus sulfation sites. Consistent with a functional role of this region of PSGL-1 in binding P-selectin, an affinity-purified polyclonal antibody against residues Gln-1-Glu-15 of PSGL-1 strongly inhibited P-selectin binding to neutrophils, whereas an antibody against residues Asp-9-Arg-23 was noninhibitory. These combined data strongly suggest that the N-terminal anionic/sulfated tyrosine motif of PSGL-1 as well as downstream sialylated carbohydrate is essential for binding of P-selectin by neutrophils."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.270.45.26734"xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.270.45.26734"xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/author | "Berndt M.C."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/author | "Berndt M.C."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/author | "Andrews R.K."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/author | "Andrews R.K."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/author | "De Luca M."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/author | "De Luca M."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/author | "Cumming D.A."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/author | "Cumming D.A."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/author | "Veldman G.M."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/author | "Veldman G.M."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/author | "Dunlop L.C."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/author | "Dunlop L.C."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/author | "Ettling R."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/author | "Ettling R."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/author | "Flannery J.V. Jr."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/author | "Flannery J.V. Jr."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7592904 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |