| http://purl.uniprot.org/citations/7596291 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7596291 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7596291 | http://www.w3.org/2000/01/rdf-schema#comment | "The Arthrobacter simplex gene coding for 3-ketosteroid-delta 1-dehydrogenase, a key enzyme in the degradation of the steroid nucleus, was cloned in Streptomyces lividans. Nucleotide sequence analysis revealed that the gene for 3-ketosteroid-delta 1-dehydrogenase (ksdD) is clustered with at least two more genes possibly involved in steroid metabolism. Upstream of ksdD, we found a gene, ksdR, encoding a hypothetical regulatory protein that shows homologies to KdgR, the negative regulator of pectin biodegradation in Erwinia, and GyIR, the activator for glycerol metabolism in Steptomyces. A helix-turn-helix DNA-binding domain can be predicted at similar positions near the N-terminal of KsdR, KdgR and GyIR. ksdl adjoining downstream to ksdD codes for a protein that has strong similarities to 3-ketosteroid-delta 5-isomerases. The highly conserved Tyr and Asp residues are present in the active-centre motif of the enzyme. The translated ksdD gene product was found to be similar to the 3-ketosteroid-delta 1-dehydrogenase of Pseudomonas testosteroni and to the fumarate reductase of Shewanella putrefaciens. A region highly conserved between the two steroid dehydrogenases can be aligned to the active-centre motif of the fumarate reductase. S. lividans strains carrying the ksdD gene overexpressed 3-ketosteroid-delta 1-dehydrogenase. The expression of 3-ketosteroid-delta 5-isomerase, however, was barely detectable in recombinant S. lividans strains carrying the ksdl gene, or in the parental Arthrobacter strain."xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2958.1995.tb02359.x"xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2958.1995.tb02359.x"xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/author | "Yamashita M."xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/author | "Yamashita M."xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/author | "Molnar I."xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/author | "Molnar I."xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/author | "Murooka Y."xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/author | "Murooka Y."xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/author | "Choi K.P."xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/author | "Choi K.P."xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/name | "Mol. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/name | "Mol. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/pages | "895-905"xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/pages | "895-905"xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/title | "Molecular cloning, expression in Streptomyces lividans, and analysis of a gene cluster from Arthrobacter simplex encoding 3-ketosteroid-delta 1-dehydrogenase, 3-ketosteroid-delta 5-isomerase and a hypothetical regulatory protein."xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/title | "Molecular cloning, expression in Streptomyces lividans, and analysis of a gene cluster from Arthrobacter simplex encoding 3-ketosteroid-delta 1-dehydrogenase, 3-ketosteroid-delta 5-isomerase and a hypothetical regulatory protein."xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/volume | "15"xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://purl.uniprot.org/core/volume | "15"xsd:string |
| http://purl.uniprot.org/citations/7596291 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7596291 |
| http://purl.uniprot.org/citations/7596291 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7596291 |