| http://purl.uniprot.org/citations/7603835 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7603835 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7603835 | http://www.w3.org/2000/01/rdf-schema#comment | "The basophilic leucaemia cell line RBL-2H3 exhibits a robust inwardly rectifying potassium current, IKIR, which is likely to be modulated by G proteins. We examined the physiological and molecular properties of this KIR conductance to define the nature of the underlying channel species. The macroscopic conductance revealed characteristics typical of classical K+ inward rectifiers of the IRK type. Channel gating was rapid, first order (tau approximately 1 ms at -100 mV) and steeply voltage dependent. Both activation potential and slope conductance were dependent on extracellular K+ concentration ([K+]o) and inward rectification persisted in the absence of internal Mg2+. The current was susceptible to a concentration- and voltage-dependent block by extracellular Na+, Cs+ and Ba2+. Initial IKIR whole-cell amplitudes as well as current rundown were dependent on the presence of 1 mM internal ATP. Perfusion of intracellular guanosine 5'-Q-(3-thiotriphosphate) (GTP[gamma S]) suppressed IKIR with an average half-time of decline of approximately 400 s. It was demonstrated that the dominant IRK-type 25 pS conductance channel was indeed suppressed by 100 microM preloaded GTP[gamma S]. Reverse transcriptase-polymerase chain reactions (RT-PCR) with RBL cell poly(A)+ RNA identified a full length K+ inward rectifier with 94% base pair homology to the recently cloned mouse IRK1 channel. It is concluded that RBL cells express a classical voltage-dependent IRK-type K+ inward rectifier RBL-IRK1 which is negatively controlled by G proteins."xsd:string |
| http://purl.uniprot.org/citations/7603835 | http://purl.org/dc/terms/identifier | "doi:10.1007/bf00374805"xsd:string |
| http://purl.uniprot.org/citations/7603835 | http://purl.org/dc/terms/identifier | "doi:10.1007/bf00374805"xsd:string |
| http://purl.uniprot.org/citations/7603835 | http://purl.uniprot.org/core/author | "Karschin A."xsd:string |
| http://purl.uniprot.org/citations/7603835 | http://purl.uniprot.org/core/author | "Karschin A."xsd:string |
| http://purl.uniprot.org/citations/7603835 | http://purl.uniprot.org/core/author | "Wischmeyer E."xsd:string |
| http://purl.uniprot.org/citations/7603835 | http://purl.uniprot.org/core/author | "Wischmeyer E."xsd:string |
| http://purl.uniprot.org/citations/7603835 | http://purl.uniprot.org/core/author | "Lentes K.U."xsd:string |
| http://purl.uniprot.org/citations/7603835 | http://purl.uniprot.org/core/author | "Lentes K.U."xsd:string |
| http://purl.uniprot.org/citations/7603835 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7603835 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7603835 | http://purl.uniprot.org/core/name | "Pflugers Arch."xsd:string |
| http://purl.uniprot.org/citations/7603835 | http://purl.uniprot.org/core/name | "Pflugers Arch."xsd:string |
| http://purl.uniprot.org/citations/7603835 | http://purl.uniprot.org/core/pages | "809-819"xsd:string |
| http://purl.uniprot.org/citations/7603835 | http://purl.uniprot.org/core/pages | "809-819"xsd:string |
| http://purl.uniprot.org/citations/7603835 | http://purl.uniprot.org/core/title | "Physiological and molecular characterization of an IRK-type inward rectifier K+ channel in a tumour mast cell line."xsd:string |
| http://purl.uniprot.org/citations/7603835 | http://purl.uniprot.org/core/title | "Physiological and molecular characterization of an IRK-type inward rectifier K+ channel in a tumour mast cell line."xsd:string |
| http://purl.uniprot.org/citations/7603835 | http://purl.uniprot.org/core/volume | "429"xsd:string |
| http://purl.uniprot.org/citations/7603835 | http://purl.uniprot.org/core/volume | "429"xsd:string |
| http://purl.uniprot.org/citations/7603835 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7603835 |
| http://purl.uniprot.org/citations/7603835 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7603835 |
| http://purl.uniprot.org/citations/7603835 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7603835 |
| http://purl.uniprot.org/citations/7603835 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7603835 |