| http://purl.uniprot.org/citations/7608167 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7608167 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7608167 | http://www.w3.org/2000/01/rdf-schema#comment | "5'-Exonuclease-2 has been purified 17,000-fold from whole cell extracts of Saccharomyces cerevisiae. A 116-kDa polypeptide parallels the enzyme activity when the purified protein is examined by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. Amino-terminal sequencing of the 116-kDa protein shows that the sequence agrees with that encoded by the HKE1 gene, previously reported to encode exonuclease-2. A 45-kDa polypeptide also parallels the enzyme activity upon purification, and Sephacryl S-200 molecular sieve chromatography of the purified enzyme shows a parallel elution of most of the 116- and 45-kDa polypeptides, suggesting a close association of the two. Enzyme instability has precluded a more detailed analysis of their associative properties. The enzyme hydrolyzes RNA substrates to 5'-mononucleotides in a processive manner. Measurements of its substrate specificity and mode of action are compared with 5'-exonuclease-1. Restriction cut single-stranded T7 DNA is hydrolyzed at approximately 5-7% of the rate of 18 S rRNA of yeast by both enzymes. That 5'-exonuclease-2 hydrolyzes in a processive manner and lacks endonuclease activity is shown by the finding that [5'-32P]GMP is the only product of its hydrolysis of [alpha-32P]GTP-labeled synthetic RNAs. That 5'-exonuclease-2 hydrolyzes by a 5'-->3' mode is shown by: 1) its poor hydrolysis of both 5'-capped and triphosphate-ended RNA substrates; 2) the products of its hydrolysis of [5'-32P,3H](pA)4; and 3) the accumulation of 3'-stall fragments when a strong artificial RNA secondary structure is present in synthetic RNAs. 5'-Exonuclease-1 hydrolyzes the synthetic RNAs and (pA)4 in an identical manner."xsd:string |
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| http://purl.uniprot.org/citations/7608167 | http://purl.uniprot.org/core/author | "Stevens A."xsd:string |
| http://purl.uniprot.org/citations/7608167 | http://purl.uniprot.org/core/author | "Stevens A."xsd:string |
| http://purl.uniprot.org/citations/7608167 | http://purl.uniprot.org/core/author | "Poole T.L."xsd:string |
| http://purl.uniprot.org/citations/7608167 | http://purl.uniprot.org/core/author | "Poole T.L."xsd:string |
| http://purl.uniprot.org/citations/7608167 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7608167 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7608167 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7608167 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7608167 | http://purl.uniprot.org/core/pages | "16063-16069"xsd:string |
| http://purl.uniprot.org/citations/7608167 | http://purl.uniprot.org/core/pages | "16063-16069"xsd:string |
| http://purl.uniprot.org/citations/7608167 | http://purl.uniprot.org/core/title | "5'-exonuclease-2 of Saccharomyces cerevisiae. Purification and features of ribonuclease activity with comparison to 5'-exonuclease-1."xsd:string |
| http://purl.uniprot.org/citations/7608167 | http://purl.uniprot.org/core/title | "5'-exonuclease-2 of Saccharomyces cerevisiae. Purification and features of ribonuclease activity with comparison to 5'-exonuclease-1."xsd:string |
| http://purl.uniprot.org/citations/7608167 | http://purl.uniprot.org/core/volume | "270"xsd:string |
| http://purl.uniprot.org/citations/7608167 | http://purl.uniprot.org/core/volume | "270"xsd:string |
| http://purl.uniprot.org/citations/7608167 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7608167 |
| http://purl.uniprot.org/citations/7608167 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7608167 |
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| http://purl.uniprot.org/citations/7608167 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7608167 |
| http://purl.uniprot.org/uniprot/Q02792 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/7608167 |
| http://purl.uniprot.org/uniprot/Q02792#attribution-B3C4A90695A98812790A6C5E17DEB454 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/7608167 |