| http://purl.uniprot.org/citations/7678348 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7678348 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7678348 | http://www.w3.org/2000/01/rdf-schema#comment | "The delayed implantation model was used to study epidermal growth factor receptor(s) (EGF-R) in the mouse blastocyst. Delayed implantation and blastocyst dormancy were induced by ovariectomy on day 4 of pregnancy and were maintained by daily (days 5-7) injections of progesterone (P4). Blastocyst activation and implantation were initiated by coinjection of estradiol-17 beta (E2) with P4 on the 3rd day of delay. Immunostaining of EGF-R, autoradiographic detection of 125I-labeled EGF binding, and measurement of EGF-inducible subcellular protein tyrosine phosphorylation demonstrated the loss of EGF-R from blastocysts (dormant) recovered 24 h after ovariectomy or on the 3rd day of P4-maintained delay. However, increased EGF-R levels were detected in blastocysts (activated) recovered 12 or 24 h after E2 injection. Blastocyst EGF-R mRNA levels were quantitated by reverse transcriptase (RT)-PCR and distribution of this mRNA was examined by in situ hybridization. To provide a homologous probe for these studies, a mouse EGF-R partial cDNA was cloned and used as the template for synthesis of antisense- and sense-strand EGF-R RNA. Quantitative RT-PCR demonstrated an 8-to 10-fold reduction in EGF-R mRNA copies per cell in dormant blastocysts. In contrast, an 8-fold increase in EGF-R mRNA copies per cell was detected in activated blastocysts 8 h after injection of E2. In situ hybridization detected EGF-R mRNA in most cells of normal day 4 blastocysts but not in those of dormant blastocysts. These studies establish that expression of the EGF-R gene in mouse blastocysts is tightly regulated by maternal steroid hormonal status."xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.90.1.55"xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.90.1.55"xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/author | "Dey S.K."xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/author | "Dey S.K."xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/author | "Das S.K."xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/author | "Das S.K."xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/author | "Paria B.C."xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/author | "Paria B.C."xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/author | "Andrews G.K."xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/author | "Andrews G.K."xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/pages | "55-59"xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/pages | "55-59"xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/title | "Expression of the epidermal growth factor receptor gene is regulated in mouse blastocysts during delayed implantation."xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/title | "Expression of the epidermal growth factor receptor gene is regulated in mouse blastocysts during delayed implantation."xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/volume | "90"xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://purl.uniprot.org/core/volume | "90"xsd:string |
| http://purl.uniprot.org/citations/7678348 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7678348 |
| http://purl.uniprot.org/citations/7678348 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7678348 |