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http://purl.uniprot.org/citations/7683678http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/7683678http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/7683678http://www.w3.org/2000/01/rdf-schema#comment"A 25-kDa protein was found to be associated with purified human neutrophil gelatinase. Polyclonal antibodies raised against gelatinase not only recognized gelatinase but also this 25-kDa protein. Specific antibodies against the 25-kDa protein were obtained by affinity purification of the gelatinase antibodies. Immunoblotting and immunoprecipitation studies demonstrated the 135-kDa form of gelatinase to be a complex of 92-kDa gelatinase and the 25-kDa protein, and the 220-kDa form was demonstrated to be a homodimer of the 92-kDa protein, thus explaining the 220-, 135-, and 92-kDa forms characteristic of neutrophil gelatinase. The 25-kDa protein was purified to apparent homogeneity from exocytosed material from phorbol myristate acetate-stimulated neutrophils. The primary structure of the 25-kDa protein was determined as a 178-residue protein. It was susceptible to treatment with N-glycanase, and one N-glycosylation site was identified. The sequence did not match any known human protein, but showed a high degree of similarity with the deduced sequences of rat alpha 2-microglobulin-related protein and the mouse protein 24p3. It is thus a new member of the lipocalin family. The function of the 25-kDa protein, named neutrophil gelatinase-associated lipocalin (NGAL), remains to be determined."xsd:string
http://purl.uniprot.org/citations/7683678http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(18)82217-7"xsd:string
http://purl.uniprot.org/citations/7683678http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(18)82217-7"xsd:string
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/author"Borregaard N."xsd:string
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/author"Borregaard N."xsd:string
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/author"Johnsen A.H."xsd:string
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/author"Johnsen A.H."xsd:string
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/author"Kjeldsen L."xsd:string
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/author"Kjeldsen L."xsd:string
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/author"Sengelov H."xsd:string
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/author"Sengelov H."xsd:string
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/date"1993"xsd:gYear
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/date"1993"xsd:gYear
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/pages"10425-10432"xsd:string
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/pages"10425-10432"xsd:string
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/title"Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase."xsd:string
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/title"Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase."xsd:string
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/volume"268"xsd:string
http://purl.uniprot.org/citations/7683678http://purl.uniprot.org/core/volume"268"xsd:string
http://purl.uniprot.org/citations/7683678http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/7683678
http://purl.uniprot.org/citations/7683678http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/7683678