| http://purl.uniprot.org/citations/7698664 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7698664 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7698664 | http://www.w3.org/2000/01/rdf-schema#comment | "We have identified and sequenced four genes that encode the protein subunits comprising the succinate dehydrogenase enzyme complex (Sdh) of the rickettsia Coxiella burnetii. The Sdh-encoding gene cluster (sdhCDAB) begins 3326 bp upstream from the citrate synthase-encoding gene (gltA) start codon and is read with opposite polarity. An open reading frame encoding the N-terminal 280 amino acids (aa) of 2-oxoglutarate dehydrogenase (SucA) begins 24 bp downstream from the stop codon of the gene specifying the iron-sulfur subunit (sdhB) of Sdh. The deduced aa sequence of Sdh subunits and the N-terminal portion of SucA revealed significant aa identity with the Esherichia coli homologues ranging from a low of 36.6% for SdhD to a high of 61.2% for SdhA and SdhB. Primer extension identified transcription start points (tsp) for sdh and sucA. The region upstream from the sdh tsp, but not the sucA tsp, displayed homology to promoter consensus sequences of E. coli. Further evidence that sucA transcription can occur independent of sdh transcription was provided by demonstrating that a TnphoA insertion disrupting sdhB had no effect on the production of SucA by an E. coli cell-extract-directed in vitro transcription/translation system. The plasmid clone pLPM60, which carries the C. burnetii sdhCDAB coding and upstream regulatory regions, rescued an E. coli sdhA mutant (MOB252), indicating functional expression of the rickettsial locus. A cell extract of MOB252 transformed with pLPM60 showed a sixfold greater level of Sdh enzyme activity over the E. coli wild type. A plasmid clone lacking the sdh upstream regulatory region did not complement nor produce sdh mRNA by dot blot analysis.(ABSTRACT TRUNCATED AT 250 WORDS)"xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.org/dc/terms/identifier | "doi:10.1016/0378-1119(94)00888-y"xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.org/dc/terms/identifier | "doi:10.1016/0378-1119(94)00888-y"xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.org/dc/terms/identifier | "doi:10.1016/0378-1119(94)00888-Y"xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/author | "Heinzen R.A."xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/author | "Heinzen R.A."xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/author | "Mallavia L.P."xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/author | "Mallavia L.P."xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/author | "Mo Y.-Y."xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/author | "Mo Y.-Y."xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/author | "Robertson S.J."xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/author | "Robertson S.J."xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/pages | "27-34"xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/pages | "27-34"xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/title | "Characterization of the succinate dehydrogenase-encoding gene cluster (sdh) from the rickettsia Coxiella burnetii."xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/title | "Characterization of the succinate dehydrogenase-encoding gene cluster (sdh) from the rickettsia Coxiella burnetii."xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/volume | "155"xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://purl.uniprot.org/core/volume | "155"xsd:string |
| http://purl.uniprot.org/citations/7698664 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7698664 |