| http://purl.uniprot.org/citations/7704278 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7704278 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7704278 | http://www.w3.org/2000/01/rdf-schema#comment | "Three Xanthobacter Py2 mutants (M3, M8 and M10) lacking epoxyalkane-degrading activity were isolated and characterized. All mutants were able to grow on acetone, the degradation product of 1,2-epoxypropane conversions. Furthermore, they contained the unidentified 'low molecular mass fraction' (LMF) necessary for epoxyalkane-degrading activity. Three cosmids from a gene bank complemented the mutation in M10 and M8 but not in mutant M3. Epoxyalkane-degrading activity in crude extracts of 1,2-epoxypropane-grown complemented mutants was similar to the wild-type activity. Surprisingly, M10 transformed with complementing cosmid pEP9 showed a constitutively expressed epoxyalkane-degrading activity, which was not observed in the wild-type strain. The cosmid pEP9 was conjugated into Xanthobacter autotrophicus GJ10, which is not able to degrade 1,2-epoxypropane. In crude extracts of X. autotrophicus GJ10(pEP9), epoxyalkane-degrading activity was demonstrated, but only after the addition of the LMF from Xanthobacter Py2. Hybridization experiments demonstrated an overlap on complementing cosmids pEP1, pEP3 and pEP9. Subcloning revealed a 4.8 kb EcoRI-HindIII fragment to be necessary for complementing the mutant M10. In the sequence of this fragment four different ORFs were found."xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.org/dc/terms/identifier | "doi:10.1099/13500872-141-2-477"xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.org/dc/terms/identifier | "doi:10.1099/13500872-141-2-477"xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/author | "van Ooyen A.J.J."xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/author | "van Ooyen A.J.J."xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/author | "de Bont J.A.M."xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/author | "de Bont J.A.M."xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/author | "Swaving J."xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/author | "Swaving J."xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/author | "Weijers C.A.G.M."xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/author | "Weijers C.A.G.M."xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/name | "Microbiology"xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/name | "Microbiology"xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/pages | "477-484"xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/pages | "477-484"xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/title | "Complementation of Xanthobacter Py2 mutants in epoxyalkane degradation; expression and nucleotide sequence of the complementing DNA fragment."xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/title | "Complementation of Xanthobacter Py2 mutants in epoxyalkane degradation; expression and nucleotide sequence of the complementing DNA fragment."xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/volume | "141"xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://purl.uniprot.org/core/volume | "141"xsd:string |
| http://purl.uniprot.org/citations/7704278 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7704278 |
| http://purl.uniprot.org/citations/7704278 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7704278 |