| http://purl.uniprot.org/citations/7731720 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7731720 | http://www.w3.org/2000/01/rdf-schema#comment | "The B-raf/c-Rmil proto-oncogene belongs to the raf/mil family of serine/threonine protein kinases. It encodes multiple protein isoforms resulting from alternative splicing of two exons located upstream of the kinase domain. Recent studies suggested that B-Raf could be the intermediate molecule between Ras and Mek-1 (MAP Kinase Kinase) in signalling pathways specific of neural cells. However, there has been no evidence for a direct interaction between B-Raf and Mek-1. We report here that different B-Raf isoforms can be co-immunoprecipitated with anti-Mek-1 antisera in COS-1 cells and that the kinase activity of B-Raf is not required for its interaction with Mek-1. We also show that all B-Raf isoforms tested phosphorylate Mek-1 in a time-dependent manner, whereas kinase defective mutants fail to do so. Finally, we demonstrate that the constitutively activated S218D, S222D and S218D/S222D mutants of Mek-1 interact similarly with B-Raf. However, only the S218D and S222D mutants, and not the S218D/S222D double mutant, can be phosphorylated by B-Raf isoforms. Therefore, serine residues 218 and 222, previously shown to regulate Mek-1 activity, appear to be the major phosphorylation sites by B-Raf in vitro."xsd:string |
| http://purl.uniprot.org/citations/7731720 | http://purl.uniprot.org/core/author | "Pouyssegur J."xsd:string |
| http://purl.uniprot.org/citations/7731720 | http://purl.uniprot.org/core/author | "Brunet A."xsd:string |
| http://purl.uniprot.org/citations/7731720 | http://purl.uniprot.org/core/author | "Papin C."xsd:string |
| http://purl.uniprot.org/citations/7731720 | http://purl.uniprot.org/core/author | "Barnier J.V."xsd:string |
| http://purl.uniprot.org/citations/7731720 | http://purl.uniprot.org/core/author | "Calothy G."xsd:string |
| http://purl.uniprot.org/citations/7731720 | http://purl.uniprot.org/core/author | "Eychene A."xsd:string |
| http://purl.uniprot.org/citations/7731720 | http://purl.uniprot.org/core/author | "Pages G."xsd:string |
| http://purl.uniprot.org/citations/7731720 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7731720 | http://purl.uniprot.org/core/name | "Oncogene"xsd:string |
| http://purl.uniprot.org/citations/7731720 | http://purl.uniprot.org/core/pages | "1647-1651"xsd:string |
| http://purl.uniprot.org/citations/7731720 | http://purl.uniprot.org/core/title | "B-Raf protein isoforms interact with and phosphorylate Mek-1 on serine residues 218 and 222."xsd:string |
| http://purl.uniprot.org/citations/7731720 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/7731720 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7731720 |
| http://purl.uniprot.org/citations/7731720 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7731720 |
| http://purl.uniprot.org/uniprot/#_P10398-mappedCitation-7731720 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7731720 |
| http://purl.uniprot.org/uniprot/#_P01116-mappedCitation-7731720 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7731720 |
| http://purl.uniprot.org/uniprot/#_P04275-mappedCitation-7731720 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7731720 |
| http://purl.uniprot.org/uniprot/#_P41240-mappedCitation-7731720 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7731720 |
| http://purl.uniprot.org/uniprot/#_P62834-mappedCitation-7731720 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7731720 |
| http://purl.uniprot.org/uniprot/#_P02751-mappedCitation-7731720 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7731720 |
| http://purl.uniprot.org/uniprot/#_P05106-mappedCitation-7731720 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7731720 |
| http://purl.uniprot.org/uniprot/#_P08514-mappedCitation-7731720 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7731720 |
| http://purl.uniprot.org/uniprot/#_P27361-mappedCitation-7731720 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7731720 |