| http://purl.uniprot.org/citations/7737999 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7737999 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7737999 | http://www.w3.org/2000/01/rdf-schema#comment | "Proteolytic processing of the transforming growth factor beta precursor (pro-TGF beta) is an essential step in the formation of the biologically active TGF beta homodimeric protein (TGF beta). The 361-amino-acid precursor pro-TGF beta 1 has within its primary structure the R-H-R-R processing signal found in many constitutively secreted precursor proteins and potentially recognized by members of the mammalian convertase family of endoproteases. To determine whether cleavage of pro-TGF beta 1 can be achieved by the furin convertase in vitro, purified precursor was incubated in the presence of a truncated/secreted form of the enzyme. Immunoblots showed that the 55-kDa pro-TGF beta 1 was converted into the 44 and 12.5 kDa bands corresponding to the pro-region and the mature monomer, respectively. Treatment of pro-TGF beta 1 with furin resulted in a 5-fold increase in the production of biologically active TGF beta 1. Furthermore, when expressed in the furin-deficient LoVo cells, no processing of pro-TGF beta 1 was observed. In contrast, efficient processing was observed when pro-TGF beta was coexpressed with the furin convertase. Collectively, these results provide evidence that in our experimental systems the TGF beta 1 precursor is efficiently and correctly processed by human furin thus permitting release of the biologically active peptide."xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.270.18.10618"xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.270.18.10618"xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/author | "Leduc R."xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/author | "Leduc R."xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/author | "Gentry L.E."xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/author | "Gentry L.E."xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/author | "Blanchette F."xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/author | "Blanchette F."xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/author | "Dubois C.M."xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/author | "Dubois C.M."xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/author | "Laprise M.H."xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/author | "Laprise M.H."xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/pages | "10618-10624"xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/pages | "10618-10624"xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/title | "Processing of transforming growth factor beta 1 precursor by human furin convertase."xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/title | "Processing of transforming growth factor beta 1 precursor by human furin convertase."xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/volume | "270"xsd:string |
| http://purl.uniprot.org/citations/7737999 | http://purl.uniprot.org/core/volume | "270"xsd:string |