| http://purl.uniprot.org/citations/7744045 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7744045 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7744045 | http://www.w3.org/2000/01/rdf-schema#comment | "The squarely arranged surface layer (S-layer) glycoprotein of Clostridium thermosaccharolyticum E207-71 was isolated from bacterial cells which were grown under defined culture conditions. By sodium dodecyl sulfate polyacrylamide gel electrophoresis, the S-layer showed a series of distinct bands with apparent molecular masses in the range 83-210 kDa. Upon deglycosylation by trifluoromethanesulfonic acid, only the single band at 83 kDa remained unchanged. After pronase digestion of the intact S-layer glycoprotein, the degradation products were isolated by gel-permeation chromatography, cation-exchange chromatography and isoelectric focusing. Three main fractions and an amino sugar containing minor fraction were obtained. The main fractions, which showed identical carbohydrate compositions, were further purified by reverse-phase chromatography and characterized by monosaccharide analysis, Smith degradation, methylation analysis, and one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy. The combined chemical and spectroscopical evidence suggest the following glycan structure for the main fractions: [Sequence: See text]"xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1995.tb20470.x"xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1995.tb20470.x"xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/author | "Messner P."xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/author | "Messner P."xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/author | "Brisson J.R."xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/author | "Brisson J.R."xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/author | "Altman E."xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/author | "Altman E."xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/author | "Schaffer C."xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/author | "Schaffer C."xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/name | "Eur J Biochem"xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/pages | "308-315"xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/pages | "308-315"xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/title | "Characterization of the glycan structure of a major glycopeptide from the surface layer glycoprotein of Clostridium thermosaccharolyticum E207-71."xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/title | "Characterization of the glycan structure of a major glycopeptide from the surface layer glycoprotein of Clostridium thermosaccharolyticum E207-71."xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/volume | "229"xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://purl.uniprot.org/core/volume | "229"xsd:string |
| http://purl.uniprot.org/citations/7744045 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7744045 |
| http://purl.uniprot.org/citations/7744045 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7744045 |