| http://purl.uniprot.org/citations/7751275 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7751275 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7751275 | http://www.w3.org/2000/01/rdf-schema#comment | "We have isolated promoters inducible by paraquat, a superoxide radical-generating agent, from Escherichia coli, using promoter-probing plasmid pJAC4 (Y.S. Koh and J.H. Roe, Korean J. Microbiol. 31:267-273, 1993). One promoter clone pqi-5 (pqi denotes paraquat-inducible gene) was mapped at 21.8 min on the E. coli chromosome by using the Kohara phage library. We constructed an operon fusion of the lacZ gene with the pqi-5 promoter to monitor the expression of the gene in the single-copy state. LacZ expression was induced about 7-to 13-fold by 77 to 780 microM paraquat. Other known superoxide generators such as menadione, phenazine methosulfate, and plumbagin also induced the expression of beta-galactosidase in this fusion strain. On the other hand, no significant induction was observed with treatment with hydrogen peroxide, ethanol, and heat shock. Induction of beta-galactosidase was significantly reduced by introducing a delta sox-8::cat or soxS3::Tn10 mutation into the fusion strain, indicating that pqi-5 is a member of the soxRS regulon. A DNA fragment containing the pqi-5 promoter was cloned and sequenced from the Kohara phage E2E5. We identified two pqi-5 open reading frames (ORFs); ORF-A encodes a predicted protein of 342 amino acids, and ORF-B is truncated at the cloning site. The transcription start site from the pqi-5 promoter was determined by primer extension and S1 nuclease protection analyses. Northern (RNA) and S1 analyses indicated that there are two kinds of pqi-5 transcript; one covers ORF-A only and the other covers ORF-A and possibly also ORF-B."xsd:string |
| http://purl.uniprot.org/citations/7751275 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.177.10.2673-2678.1995"xsd:string |
| http://purl.uniprot.org/citations/7751275 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.177.10.2673-2678.1995"xsd:string |
| http://purl.uniprot.org/citations/7751275 | http://purl.uniprot.org/core/author | "Koh Y.-S."xsd:string |
| http://purl.uniprot.org/citations/7751275 | http://purl.uniprot.org/core/author | "Koh Y.-S."xsd:string |
| http://purl.uniprot.org/citations/7751275 | http://purl.uniprot.org/core/author | "Roe J.-H."xsd:string |
| http://purl.uniprot.org/citations/7751275 | http://purl.uniprot.org/core/author | "Roe J.-H."xsd:string |
| http://purl.uniprot.org/citations/7751275 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7751275 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7751275 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/7751275 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/7751275 | http://purl.uniprot.org/core/pages | "2673-2678"xsd:string |
| http://purl.uniprot.org/citations/7751275 | http://purl.uniprot.org/core/pages | "2673-2678"xsd:string |
| http://purl.uniprot.org/citations/7751275 | http://purl.uniprot.org/core/title | "Isolation of a novel paraquat-inducible (pqi) gene regulated by the soxRS locus in Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/7751275 | http://purl.uniprot.org/core/title | "Isolation of a novel paraquat-inducible (pqi) gene regulated by the soxRS locus in Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/7751275 | http://purl.uniprot.org/core/volume | "177"xsd:string |
| http://purl.uniprot.org/citations/7751275 | http://purl.uniprot.org/core/volume | "177"xsd:string |
| http://purl.uniprot.org/citations/7751275 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7751275 |
| http://purl.uniprot.org/citations/7751275 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7751275 |
| http://purl.uniprot.org/citations/7751275 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7751275 |
| http://purl.uniprot.org/citations/7751275 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7751275 |
| http://purl.uniprot.org/uniprot/P0AFL9 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/7751275 |
| http://purl.uniprot.org/uniprot/P43672 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/7751275 |