| http://purl.uniprot.org/citations/7751634 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7751634 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7751634 | http://www.w3.org/2000/01/rdf-schema#comment | "We previously reported that the Gram-negative bacterium Yersinia pseudotuberculosis produces a superantigen (YPM, Y. pseudotuberculosis-derived mitogen) that expands T cells bearing V betas 3, 9, 13.1, and 13.2 in an MHC class II-dependent manner. Based on the previously determined N-terminal 23 amino acids of YPM (T-D-Y-D-N-T-L-N-S-I-P-S-L-R-I-P-N-I-A-T-Y-T-G-(one-letter code)), we cloned the ypm gene and analyzed the nucleotide sequence. The gene encodes a 151-amino acid protein with a 20-amino acid signal peptide at its N terminus. The recombinant YPM expressed by the cloned gene exerted a mitogenic activity on human PBMC at a concentration of approximately 1 pg/ml. T cells bearing V beta 13.3 were preferentially expanded as well as T cells bearing the same V beta repertoires stimulated by native YPM. T cells were stimulated by the recombinant YPM in the presence of either fixed or unfixed HLA class II-transfected mouse fibroblasts. Furthermore, sequence diversity in the junctional region of the TCR beta-chain containing the V beta 3 element could be observed after stimulation by the recombinant YPM. These results indicate that YPM belongs to the category of superantigens and should be included as a novel member. The amino acid sequence of the mature protein showed no significant homology to other superantigens derived from Gram-positive bacteria such as Staphylococcus aureus and Streptococcus pyogenes. This observation, together with the substantially smaller m.w. suggest that ypm must have evolved from a different ancestral gene."xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/author | "Ito Y."xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/author | "Ito Y."xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/author | "Takeda T."xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/author | "Takeda T."xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/author | "Yoshino K."xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/author | "Yoshino K."xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/author | "Abe J."xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/author | "Abe J."xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/author | "Kohsaka T."xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/author | "Kohsaka T."xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/name | "J. Immunol."xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/pages | "5896-5906"xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/pages | "5896-5906"xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/title | "Sequence analysis of the gene for a novel superantigen produced by Yersinia pseudotuberculosis and expression of the recombinant protein."xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/title | "Sequence analysis of the gene for a novel superantigen produced by Yersinia pseudotuberculosis and expression of the recombinant protein."xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/volume | "154"xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://purl.uniprot.org/core/volume | "154"xsd:string |
| http://purl.uniprot.org/citations/7751634 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7751634 |
| http://purl.uniprot.org/citations/7751634 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7751634 |