| http://purl.uniprot.org/citations/7760844 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7760844 | http://www.w3.org/2000/01/rdf-schema#comment | "Using the 3T3-F442A preadipocyte line as a model of GH-dependent differentiation, early changes in the DNA-binding affinity of transcription factors in response to GH addition were investigated. Addition of 50 ng/ml human GH to cells in chemically defined medium led to a rapid increase in binding activity of activator protein 1 (AP-1) and CCAAT enhancer-binding protein (C/EBP), which was significant at 30 min and reached maximal induction by 2 h (3-fold for AP-1, 2.5-fold for C/EBP). Induction in AP-1 DNA binding correlates with a concomitant GH trans-activation of c-jun and c-fos genes described previously. Using specific antibodies in electrophoretic mobility shift assays and Western blots, it was shown that the increase in activity of C/EBP is the result of an increase in synthesis of two alternatively translated forms of C/EBP beta: 40-C/EBP beta and 23-C/EBP beta. This increase in protein was not accompanied by alteration in mRNA level and could be blocked by a Janus kinase 2 tyrosine kinase inhibitor and a C kinase inhibitor at concentrations shown to inhibit GH-dependent activation of microtubule-associated protein (MAP) kinases. Concomitant with the translationally activated increase in C/EBP beta, a GH-dependent increase was observed in C/EBP delta transcription. This was accompanied by an increase in mRNA for C/EBP delta, which was superinduced by cycloheximide and, unlike the increase in C/EBP beta protein, was not observed with insulin. Thus GH exerts its effects on C/EBP isoforms at two levels: transcriptional activation of C/EBP delta and translational activation of C/EBP beta. It is proposed that GH-dependent phosphorylation results in the efficient translation of 40-C/EBP beta and 23-C/EBP beta (the mouse homolog of the inhibitor liver-enriched inhibitory protein), and that together with the induction of C/EBP delta, these may be involved in initiating the adipocyte differentiation program."xsd:string |
| http://purl.uniprot.org/citations/7760844 | http://purl.org/dc/terms/identifier | "doi:10.1210/mend.9.1.7760844"xsd:string |
| http://purl.uniprot.org/citations/7760844 | http://purl.uniprot.org/core/author | "Wells C."xsd:string |
| http://purl.uniprot.org/citations/7760844 | http://purl.uniprot.org/core/author | "Chen C.M."xsd:string |
| http://purl.uniprot.org/citations/7760844 | http://purl.uniprot.org/core/author | "Muscat G.E."xsd:string |
| http://purl.uniprot.org/citations/7760844 | http://purl.uniprot.org/core/author | "Harrison S."xsd:string |
| http://purl.uniprot.org/citations/7760844 | http://purl.uniprot.org/core/author | "Waters M.J."xsd:string |
| http://purl.uniprot.org/citations/7760844 | http://purl.uniprot.org/core/author | "Clarkson R.W."xsd:string |
| http://purl.uniprot.org/citations/7760844 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7760844 | http://purl.uniprot.org/core/name | "Mol Endocrinol"xsd:string |
| http://purl.uniprot.org/citations/7760844 | http://purl.uniprot.org/core/pages | "108-120"xsd:string |
| http://purl.uniprot.org/citations/7760844 | http://purl.uniprot.org/core/title | "Early responses of trans-activating factors to growth hormone in preadipocytes: differential regulation of CCAAT enhancer-binding protein-beta (C/EBP beta) and C/EBP delta."xsd:string |
| http://purl.uniprot.org/citations/7760844 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/7760844 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7760844 |
| http://purl.uniprot.org/citations/7760844 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7760844 |
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| http://purl.uniprot.org/uniprot/#_B9EIA9-mappedCitation-7760844 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7760844 |
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| http://purl.uniprot.org/uniprot/#_Q00322-mappedCitation-7760844 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7760844 |
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| http://purl.uniprot.org/uniprot/#_Q3UPN9-mappedCitation-7760844 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7760844 |
| http://purl.uniprot.org/uniprot/#_Q3U0K9-mappedCitation-7760844 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7760844 |