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http://purl.uniprot.org/citations/7762638http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/7762638http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/7762638http://www.w3.org/2000/01/rdf-schema#comment"The cDNA clone encoding the fast-twitch isoform of myosin light chain 1 (MLC-1f) was isolated from bovine longissimus dorsi muscle and sequenced in M13 and pUC8. An 0.8-kb subclone, produced by digestion of the cDNA with EcoRI, contained the portion of the molecule common to MLC-1f and MLC-3f. The cDNA in pUC8 contained an additional 81 bp upstream of the EcoR I digestion site, which was unique to MLC-1f. The cDNA clone was used to measure MLC-1f mRNA in longissimus dorsi muscle of cattle chronically administered the beta-adrenergic agonist clenbuterol. Treatment with clenbuterol for 50 days increased succinic dehydrogenase negative (type IIB) and positive (types I and IIA) myofiber cross-sectional areas by 25%. After the 50-day treatment period, the amount of MLC-1f mRNA was 90% greater in longissimus dorsi muscle of treated animals than in the initial group. This effect was lost when clenbuterol treatment was withdrawn for a 78-day period, during which time muscle growth in the treated animals stopped completely. We conclude that we have cloned the bovine cDNA for MLC-1f, which has provided additional evidence that beta-adrenergic agonists increase myofibrillar gene expression."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.org/dc/terms/identifier"doi:10.1152/ajpendo.1995.268.5.e858"xsd:string
http://purl.uniprot.org/citations/7762638http://purl.org/dc/terms/identifier"doi:10.1152/ajpendo.1995.268.5.e858"xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/author"Garcia D.K."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/author"Garcia D.K."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/author"Wu F.Y."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/author"Wu F.Y."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/author"Smith S.B."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/author"Smith S.B."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/author"Davis S.K."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/author"Davis S.K."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/author"Wilson J.J."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/author"Wilson J.J."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/author"Stone R.T."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/author"Stone R.T."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/author"Lunt D.K."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/author"Lunt D.K."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/author"Schiavetta A.M."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/author"Schiavetta A.M."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/date"1995"xsd:gYear
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/date"1995"xsd:gYear
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/name"Am. J. Physiol."xsd:string
http://purl.uniprot.org/citations/7762638http://purl.uniprot.org/core/name"Am. J. Physiol."xsd:string