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http://purl.uniprot.org/citations/7763659http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/7763659http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/7763659http://www.w3.org/2000/01/rdf-schema#comment"Three chitinases, designated RSC-a, -b, and -c, were purified from the seeds of rye (Secale cereal) using ammonium sulfate precipitation, CM-cellulose column chromatography, gel filtration on Sephadex G-75, and S-Sepharose column chromatography. RSC-a, -b, and -c are basic proteins having molecular masses of 33 kDa, 26 kDa, and 26 kDa, and isoelectric points of 9.7, 10, and > 10, respectively. RSC-b and -c were found to be homologous proteins having similar amino acid compositions and N-terminal sequences. RSC-a contains more Thr, Ser, Glu, Pro, Gly, and Cys than RSC-b and -c and has a different N-terminal sequence from them. They hydrolyze glycolchitin and colloidal chitin, but not cell walls of Micrococcus lysodeikticus. These enzymes are stable at pH 4-8 and their optimum pHs toward glycolchitin are 5."xsd:string
http://purl.uniprot.org/citations/7763659http://purl.org/dc/terms/identifier"doi:10.1271/bbb.57.643"xsd:string
http://purl.uniprot.org/citations/7763659http://purl.org/dc/terms/identifier"doi:10.1271/bbb.57.643"xsd:string
http://purl.uniprot.org/citations/7763659http://purl.uniprot.org/core/author"Yamagami T."xsd:string
http://purl.uniprot.org/citations/7763659http://purl.uniprot.org/core/author"Yamagami T."xsd:string
http://purl.uniprot.org/citations/7763659http://purl.uniprot.org/core/author"Funatsu G."xsd:string
http://purl.uniprot.org/citations/7763659http://purl.uniprot.org/core/author"Funatsu G."xsd:string
http://purl.uniprot.org/citations/7763659http://purl.uniprot.org/core/date"1993"xsd:gYear
http://purl.uniprot.org/citations/7763659http://purl.uniprot.org/core/date"1993"xsd:gYear
http://purl.uniprot.org/citations/7763659http://purl.uniprot.org/core/name"Biosci. Biotechnol. Biochem."xsd:string
http://purl.uniprot.org/citations/7763659http://purl.uniprot.org/core/name"Biosci. Biotechnol. Biochem."xsd:string
http://purl.uniprot.org/citations/7763659http://purl.uniprot.org/core/pages"643-647"xsd:string
http://purl.uniprot.org/citations/7763659http://purl.uniprot.org/core/pages"643-647"xsd:string
http://purl.uniprot.org/citations/7763659http://purl.uniprot.org/core/title"Purification and some properties of three chitinases from the seeds of rye (Secale cereale)."xsd:string
http://purl.uniprot.org/citations/7763659http://purl.uniprot.org/core/title"Purification and some properties of three chitinases from the seeds of rye (Secale cereale)."xsd:string
http://purl.uniprot.org/citations/7763659http://purl.uniprot.org/core/volume"57"xsd:string
http://purl.uniprot.org/citations/7763659http://purl.uniprot.org/core/volume"57"xsd:string
http://purl.uniprot.org/citations/7763659http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/7763659
http://purl.uniprot.org/citations/7763659http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/7763659
http://purl.uniprot.org/citations/7763659http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/7763659
http://purl.uniprot.org/citations/7763659http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/7763659
http://purl.uniprot.org/uniprot/Q9FRV1http://purl.uniprot.org/core/citationhttp://purl.uniprot.org/citations/7763659
http://purl.uniprot.org/uniprot/Q9FRV0http://purl.uniprot.org/core/citationhttp://purl.uniprot.org/citations/7763659