| http://purl.uniprot.org/citations/7768825 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7768825 | http://www.w3.org/2000/01/rdf-schema#comment | "The kinetics of glucose uptake in Saccharomyces cerevisiae are complex. An Eadie-Hofstee (rate of uptake versus rate of uptake over substrate concentration) plot of glucose uptake shows a nonlinear form typical of a multicomponent system. The nature of the constituent components is a subject of debate. It has recently been suggested that this nonlinearity is due to either a single saturable component together with free diffusion of glucose or a single constitutive component with a variable Km, rather than the action of multiple hexose transporters. Genetic data support the existence of a family of differentially regulated glucose transporters, encoded by the HXT genes. In this work, kinetic expressions and nonlinear regression analysis, based on an improved zero trans-influx assay, were used to address the nature of the components of the transport system. The results indicate that neither one component with free diffusion nor a single permease with a variable Km can explain the observed uptake rates. Results of uptake experiments, including the use of putative alternative substrates as inhibitory compounds, support the model derived from genetic analyses of a multicomponent system with at least two components, one a high-affinity carrier and the other a low-affinity carrier. This approach was extended to characterize the activity of the SNF3 protein and identify its role in the depression of high-affinity uptake. The kinetic data support a role of SNF3 as a regulatory protein that may not itself be a transporter."xsd:string |
| http://purl.uniprot.org/citations/7768825 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.177.11.3251-3258.1995"xsd:string |
| http://purl.uniprot.org/citations/7768825 | http://purl.uniprot.org/core/author | "Bisson L.F."xsd:string |
| http://purl.uniprot.org/citations/7768825 | http://purl.uniprot.org/core/author | "Coons D.M."xsd:string |
| http://purl.uniprot.org/citations/7768825 | http://purl.uniprot.org/core/author | "Boulton R.B."xsd:string |
| http://purl.uniprot.org/citations/7768825 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7768825 | http://purl.uniprot.org/core/name | "J Bacteriol"xsd:string |
| http://purl.uniprot.org/citations/7768825 | http://purl.uniprot.org/core/pages | "3251-3258"xsd:string |
| http://purl.uniprot.org/citations/7768825 | http://purl.uniprot.org/core/title | "Computer-assisted nonlinear regression analysis of the multicomponent glucose uptake kinetics of Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/7768825 | http://purl.uniprot.org/core/volume | "177"xsd:string |
| http://purl.uniprot.org/citations/7768825 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7768825 |
| http://purl.uniprot.org/citations/7768825 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7768825 |
| http://purl.uniprot.org/uniprot/#_P10870-mappedCitation-7768825 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7768825 |
| http://purl.uniprot.org/uniprot/P10870 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/7768825 |