| http://purl.uniprot.org/citations/7785982 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7785982 | http://www.w3.org/2000/01/rdf-schema#comment | "Oryzacystatin (OC) is the first-described cystatin originating from rice seed; it consists of two molecular species, OC-I and OC-II, which have antiviral action against poliovirus in vitro (H. Kondo, S. Ijiri, K. Abe, H. Maeda, and S. Arai, FEBS Lett. 299:48-50, 1992). In the experiments reported here, we investigated the effects of OC-I and OC-II on the replication of herpes simplex virus type 1 (HSV-1) in vitro and in vivo. HSV-1 was inoculated onto monolayers of monkey kidney epithelial cells (CV-1 cells) at a multiplicity of infection of 0.1 PFU per cell. After adsorption of the virus onto cells, the cultures were incubated in the presence of either OC-I or OC-II in the concentration range of 1.0 to 300 microM, and the supernatant virus yield was quantitated at 24 h. The effective concentration for 90% inhibition of HSV-1 was 14.8 microM, while a cytotoxic effect on CV-1 cells without infection of HSV-1 was not observed below 500 microM OC-I. Therefore, the apparent in vitro chemotherapeutic index was estimated to be more than 33. In the mouse model of HSV-1-induced keratitis and encephalopathy, topical administration of OC-I to the mouse cornea produced a significant decrease in virus production in the cornea (mean virus yields: 3.11 log10 PFU in the treated group and 4.37 log10 PFU in the control group) and significant improvement in survival rates (P = 0.01). The in vivo antiherpetic effect of OC-I was comparable to that of acyclovir, indicating that topical treatment of HSV-1 infection in humans with OC-I might be possible. Our data also suggest the importance of some thiol proteinases, which may be derived from either the host's cells or HSV-1, during the replication process of HSV-1."xsd:string |
| http://purl.uniprot.org/citations/7785982 | http://purl.org/dc/terms/identifier | "doi:10.1128/aac.39.4.846"xsd:string |
| http://purl.uniprot.org/citations/7785982 | http://purl.uniprot.org/core/author | "Abe K."xsd:string |
| http://purl.uniprot.org/citations/7785982 | http://purl.uniprot.org/core/author | "Arai S."xsd:string |
| http://purl.uniprot.org/citations/7785982 | http://purl.uniprot.org/core/author | "Aoki H."xsd:string |
| http://purl.uniprot.org/citations/7785982 | http://purl.uniprot.org/core/author | "Kuroda M."xsd:string |
| http://purl.uniprot.org/citations/7785982 | http://purl.uniprot.org/core/author | "Maeda H."xsd:string |
| http://purl.uniprot.org/citations/7785982 | http://purl.uniprot.org/core/author | "Akaike T."xsd:string |
| http://purl.uniprot.org/citations/7785982 | http://purl.uniprot.org/core/author | "Negi A."xsd:string |
| http://purl.uniprot.org/citations/7785982 | http://purl.uniprot.org/core/author | "Okamura R."xsd:string |
| http://purl.uniprot.org/citations/7785982 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7785982 | http://purl.uniprot.org/core/name | "Antimicrob Agents Chemother"xsd:string |
| http://purl.uniprot.org/citations/7785982 | http://purl.uniprot.org/core/pages | "846-849"xsd:string |
| http://purl.uniprot.org/citations/7785982 | http://purl.uniprot.org/core/title | "Antiviral effect of oryzacystatin, a proteinase inhibitor in rice, against herpes simplex virus type 1 in vitro and in vivo."xsd:string |
| http://purl.uniprot.org/citations/7785982 | http://purl.uniprot.org/core/volume | "39"xsd:string |
| http://purl.uniprot.org/citations/7785982 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7785982 |
| http://purl.uniprot.org/citations/7785982 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7785982 |
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| http://purl.uniprot.org/uniprot/#_A2ZYR4-mappedCitation-7785982 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7785982 |
| http://purl.uniprot.org/uniprot/#_P09229-mappedCitation-7785982 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7785982 |
| http://purl.uniprot.org/uniprot/#_P20907-mappedCitation-7785982 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7785982 |
| http://purl.uniprot.org/uniprot/P20907 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/7785982 |
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