| http://purl.uniprot.org/citations/7798208 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7798208 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7798208 | http://www.w3.org/2000/01/rdf-schema#comment | "The human immunodeficiency virus, type 1 (HIV-1) genome encodes a 15-kDa accessory gene product, Vpr, that is essential for virus replication in primary monocytes/macrophages. Being present in the virion, Vpr is believed to function in the early phases of HIV-1 replication, including nuclear migration of the pre-integration complex and/or transcription of the provirus genome. By gel filtration analysis of highly purified Vpr protein and its mutants, we demonstrate that HIV-1 Vpr exists as an oligomer. The N-terminal domain of Vpr (amino acids (aa) 1-42) is sufficient for oligomerization; however, deletion of aa 36-76 from Vpr disrupts oligomerization, suggesting that aa 36-42 are critical for Vpr oligomerization. As a result of Vpr oligomerization, basic aa residues within Vpr aa 1-73 are highly resistant to trypsin digestion, while those within Vpr aa 74-96 are easily accessible. Mutations within the leucine-/isoleucine-rich domain (aa 60-81), which was previously identified to be involved in Vpr interaction with a host cellular protein, rendered Arg62 more susceptible to trypsin digestion. Thus, the Vpr oligomeric structure must be extended into this domain. These results suggest a novel feature of HIV-1 Vpr that may be important for its functions."xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)31610-7"xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)31610-7"xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/author | "Wang L."xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/author | "Wang L."xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/author | "Mukherjee S."xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/author | "Mukherjee S."xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/author | "Zhao L.J."xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/author | "Zhao L.J."xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/author | "Narayan O."xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/author | "Narayan O."xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/pages | "32131-32137"xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/pages | "32131-32137"xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/title | "Biochemical mechanism of HIV-1 Vpr function. Oligomerization mediated by the N-terminal domain."xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/title | "Biochemical mechanism of HIV-1 Vpr function. Oligomerization mediated by the N-terminal domain."xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/volume | "269"xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://purl.uniprot.org/core/volume | "269"xsd:string |
| http://purl.uniprot.org/citations/7798208 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7798208 |
| http://purl.uniprot.org/citations/7798208 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7798208 |