| http://purl.uniprot.org/citations/7803403 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7803403 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7803403 | http://www.w3.org/2000/01/rdf-schema#comment | "The three-dimensional solution structure of PMP-D2, a 35 amino acid peptide isolated from the insect Locusta migratoria, has been determined from two-dimensional 1H NMR spectroscopy data. The structure calculations were performed from 222 NOE-derived interproton distances and 11 dihedral angles calculated from the JHN-H alpha coupling constants, using either a combination of distance geometry and restrained simulated annealing or by restrained simulated annealing alone. PMP-D2 contains three disulfide bridges that have been assigned from NMR data and structure calculations and independently confirmed using chemical and enzymatic methods. The core region of PMP-D2 adopts a compact globular fold, stabilized by hydrophobic interactions, which consists of a short three-stranded antiparallel beta-sheet involving residues 8-11, 15-19, and 25-29. Back-calculation of the NOESY spectra was used to validate the final structures. Analysis of the CD spectra of PMP-D2 under various conditions of ionic strength and in the presence of organic solvents demonstrates the high stability of this molecule. PMP-D2 was recently shown to inhibit Ca2+ currents. This activity is discussed based on the comparison of PMP-D2 three-dimensional structure with the recently established three-dimensional structure of the Ca2+ channel blocker omega-conotoxin GVIA."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00255a021"xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00255a021"xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "van Dorsselaer A."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "van Dorsselaer A."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "Kellenberger C."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "Kellenberger C."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "Luu B."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "Luu B."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "Sorokine O."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "Sorokine O."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "Hietter H."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "Hietter H."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "Mer G."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "Mer G."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "Koehl P."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "Koehl P."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "Lefevre J.-F."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "Lefevre J.-F."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "Stote R."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/author | "Stote R."xsd:string |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/7803403 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |