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http://purl.uniprot.org/citations/7805866http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/7805866http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/7805866http://www.w3.org/2000/01/rdf-schema#comment"The Na(+)-translocating NADH-quinone reductase purified from the marine bacterium Vibrio alginolyticus is composed of three subunits, alpha, beta and gamma. From the N-terminal amino acid sequences of each subunit and its polypeptide fragment obtained by partial digestion with V8 protease, oligonucleotides corresponding to forward and reverse primers for each gene (NQR A, B and C) encoding the alpha, beta and gamma subunit, respectively, were synthesized. Using these primers, a part of each gene was amplified from the chromosomal DNA of V. alginolyticus by a PCR method, and the PCR products were used for the cloning of the NQR gene in lambda phage. Among the subclones selected by probe C, the expression of the beta-subunit as a gene product was detected in Escherichia coli membranes by activity staining and Western blotting."xsd:string
http://purl.uniprot.org/citations/7805866http://purl.org/dc/terms/identifier"doi:10.1016/0014-5793(94)01274-1"xsd:string
http://purl.uniprot.org/citations/7805866http://purl.org/dc/terms/identifier"doi:10.1016/0014-5793(94)01274-1"xsd:string
http://purl.uniprot.org/citations/7805866http://purl.uniprot.org/core/author"Hayashi M."xsd:string
http://purl.uniprot.org/citations/7805866http://purl.uniprot.org/core/author"Hayashi M."xsd:string
http://purl.uniprot.org/citations/7805866http://purl.uniprot.org/core/author"Hirai K."xsd:string
http://purl.uniprot.org/citations/7805866http://purl.uniprot.org/core/author"Hirai K."xsd:string
http://purl.uniprot.org/citations/7805866http://purl.uniprot.org/core/author"Unemoto T."xsd:string
http://purl.uniprot.org/citations/7805866http://purl.uniprot.org/core/author"Unemoto T."xsd:string
http://purl.uniprot.org/citations/7805866http://purl.uniprot.org/core/date"1994"xsd:gYear
http://purl.uniprot.org/citations/7805866http://purl.uniprot.org/core/date"1994"xsd:gYear
http://purl.uniprot.org/citations/7805866http://purl.uniprot.org/core/name"FEBS Lett."xsd:string
http://purl.uniprot.org/citations/7805866http://purl.uniprot.org/core/name"FEBS Lett."xsd:string
http://purl.uniprot.org/citations/7805866http://purl.uniprot.org/core/pages"330-332"xsd:string
http://purl.uniprot.org/citations/7805866http://purl.uniprot.org/core/pages"330-332"xsd:string
http://purl.uniprot.org/citations/7805866http://purl.uniprot.org/core/title"Cloning of the Na(+)-translocating NADH-quinone reductase gene from the marine bacterium Vibrio alginolyticus and the expression of the beta-subunit in Escherichia coli."xsd:string
http://purl.uniprot.org/citations/7805866http://purl.uniprot.org/core/title"Cloning of the Na(+)-translocating NADH-quinone reductase gene from the marine bacterium Vibrio alginolyticus and the expression of the beta-subunit in Escherichia coli."xsd:string
http://purl.uniprot.org/citations/7805866http://purl.uniprot.org/core/volume"356"xsd:string
http://purl.uniprot.org/citations/7805866http://purl.uniprot.org/core/volume"356"xsd:string
http://purl.uniprot.org/citations/7805866http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/7805866
http://purl.uniprot.org/citations/7805866http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/7805866
http://purl.uniprot.org/citations/7805866http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/7805866
http://purl.uniprot.org/citations/7805866http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/7805866