| http://purl.uniprot.org/citations/782527 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/782527 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/782527 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/782527 | http://www.w3.org/2000/01/rdf-schema#comment | "A sensitive and quantitative assay for 3-octaprenyl-4-hydroxybenzoate carboxy-lyase has been developed. This enzyme, which catalyses the third reaction in ubiquinone biosynthesis in Escherichia coli, was partially purified and some of its properties determined. It was found that a considerable proportion of the carboxylyase activity could be separated from the membrane fraction in cell extracts prepared using a French press. Gel filtration showed the molecular weight of the enzyme to be about 340 000. For optimal activity the carboxy-lase was shown to require Mn2+, washed membranes or an extract of phospholipids, and an unidentified heat stable factor of molecular weight less than 10 000. The carboxy-lyase reaction was also shown to be strongly stimulated by dithiothreitol and methanol. The properties of the carboxy-lyase are compared with the three other enzymes concerned with ubiquinone biosynthesis in E. coli which have been studied in vitro. The fact that the substrate of the carboxy-lyase is membrane-bound and the enzyme is stimulated by phospholipid suggests that it normally functions in association with the cytoplasmic membrane in vivo."xsd:string |
| http://purl.uniprot.org/citations/782527 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/782527 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/782527 | http://purl.org/dc/terms/identifier | "doi:10.1016/0005-2736(76)90407-7"xsd:string |
| http://purl.uniprot.org/citations/782527 | http://purl.org/dc/terms/identifier | "doi:10.1016/0005-2736(76)90407-7"xsd:string |
| http://purl.uniprot.org/citations/782527 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/782527 |
| http://purl.uniprot.org/citations/782527 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/782527 |
| http://purl.uniprot.org/citations/782527 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/782527 |
| http://purl.uniprot.org/citations/782527 | http://purl.uniprot.org/core/author | "Gibson F."xsd:string |
| http://purl.uniprot.org/citations/782527 | http://purl.uniprot.org/core/author | "Gibson F."xsd:string |
| http://purl.uniprot.org/citations/782527 | http://purl.uniprot.org/core/author | "Young I.G."xsd:string |
| http://purl.uniprot.org/citations/782527 | http://purl.uniprot.org/core/author | "Young I.G."xsd:string |
| http://purl.uniprot.org/citations/782527 | http://purl.uniprot.org/core/author | "Leppik R.A."xsd:string |
| http://purl.uniprot.org/citations/782527 | http://purl.uniprot.org/core/author | "Leppik R.A."xsd:string |
| http://purl.uniprot.org/citations/782527 | http://purl.uniprot.org/core/date | "1976"xsd:gYear |
| http://purl.uniprot.org/citations/782527 | http://purl.uniprot.org/core/date | "1976"xsd:gYear |
| http://purl.uniprot.org/citations/782527 | http://purl.uniprot.org/core/pages | "800-810"xsd:string |
| http://purl.uniprot.org/citations/782527 | http://purl.uniprot.org/core/pages | "800-810"xsd:string |
| http://purl.uniprot.org/citations/782527 | http://purl.uniprot.org/core/title | "Membrane-associated reactions in ubiquinone biosynthesis in Escherichia coli. 3-Octaprenyl-4-hydroxybenzoate carboxy-lyase."xsd:string |
| http://purl.uniprot.org/citations/782527 | http://purl.uniprot.org/core/title | "Membrane-associated reactions in ubiquinone biosynthesis in Escherichia coli. 3-Octaprenyl-4-hydroxybenzoate carboxy-lyase."xsd:string |
| http://purl.uniprot.org/citations/782527 | http://purl.uniprot.org/core/volume | "436"xsd:string |
| http://purl.uniprot.org/citations/782527 | http://purl.uniprot.org/core/volume | "436"xsd:string |